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Team:Hawaii/Notebook/2008-06-19
From 2008.igem.org
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:<strong> Krystle & Margaret </strong> | :<strong> Krystle & Margaret </strong> | ||
| + | :* Plasmid prep was performed according to the experimental protocol | ||
:* Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101). | :* Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101). | ||
| + | |||
===Cryo-Stocks=== | ===Cryo-Stocks=== | ||
:<strong>Krystle</strong> | :<strong>Krystle</strong> | ||
:* Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024. | :* Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024. | ||
| + | |||
| + | ===Run Gel for pRL1383a plasmid Prep=== | ||
| + | :<strong>Norman</strong> | ||
| + | :*See experiment for details. | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 04:32, 20 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Alkaline Lysis Miniprep
- Krystle & Margaret
- Plasmid prep was performed according to the experimental protocol
- Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101).
Cryo-Stocks
- Krystle
- Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
Run Gel for pRL1383a plasmid Prep
- Norman
- See experiment for details.
Drylab Work
Primer Discussion
- Krystle, Norman, & Margaret
- Discussed the format of primers
- worked on revising primers
Discussion
Quote of the Day
Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens. - KS
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
