Team:Warsaw/Calendar-Main/15 October 2008
From 2008.igem.org
(Difference between revisions)
Line 13: | Line 13: | ||
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3> | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3> | ||
<h4>Piotr, Michał K.</h4> | <h4>Piotr, Michał K.</h4> | ||
- | <ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from 30 September).</li> | + | <ol><li> Repetition of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of isolated DNA fragments: <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a> (from <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/30_September_2008>30 September</a>).</li> |
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with above ligation.</li> | ||
<li> Plating on LB with kanamycin.</li></ol> | <li> Plating on LB with kanamycin.</li></ol> |
Latest revision as of 23:46, 27 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009). Preparation of OmpA-linker-omega-linker (BBa_K103016)Piotr, Michał K.
Preparation of AID under pBAD/araC (BBa_K103002)Michał K.Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin. Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)Michał K.Inoculation of colonies from plate with ligation of pSB2K3 (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin. |