Team:LCG-UNAM-Mexico/Parts
From 2008.igem.org
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<br /> </td> | <br /> </td> | ||
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- | <td colspan="4" valign="top"><img src="https://static.igem.org/mediawiki/2008/7/70/Head_spacer.gif" alt="" width="305" height="1" border="0" /><br /> | + | <td colspan="4" valign="top"><div align="center"><img src="https://static.igem.org/mediawiki/2008/7/70/Head_spacer.gif" alt="" width="305" height="1" border="0" /><br /> |
+ | <a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a> <a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /><br /> | ||
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- | + | </div> | |
- | + | <table border="0" cellspacing="0" cellpadding="2" width="590"> | |
<tr> | <tr> | ||
- | <td colspan="4" class="pageName">< | + | <td colspan="4" class="pageName"><div align="center">Parts Submitted to the Registry </div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="4" class="pageName">Constructed Parts</td> | + | <td colspan="4" class="pageName"><div align="center"><br> |
+ | <span class="style1">Constructed Parts</span></div></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="4" class="bodyText">The following are the bioparts that we | + | <td colspan="4" class="bodyText">The following are the bioparts that we designed for our project:</td> |
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- | <td colspan="3" class="calHeader">Regulatory Parts</td> | + | <td colspan="3" class="calHeader style2"><a name="regparts"></a>Regulatory Parts</td> |
<td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left | <td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left | ||
"></td> | "></td> | ||
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<td colspan="2" class="subHeader">Description:</td> | <td colspan="2" class="subHeader">Description:</td> | ||
- | <td colspan="2" class="bodyText">LacZ constitutive promoter, without | + | <td colspan="2" class="bodyText">LacZ constitutive promoter, without operators. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker</td> | + | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker</div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" class="bodyText">Operator for RcnR(YohL) the | + | <td colspan="2" class="bodyText">Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in <i>E.coli</i> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="3" class="calHeader">Coding Parts</td> | + | <td colspan="3" class="calHeader style2"><a name="codparts"></a>Coding Parts</td> |
<td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left | <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left | ||
"></td> | "></td> | ||
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<td colspan="2" class="subHeader">Designed by:</td> | <td colspan="2" class="subHeader">Designed by:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td> | + | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="3" class="calHeader">Primers</td> | + | <td colspan="3" class="calHeader style2"><a name="primers"></a>Primers</td> |
<td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left | <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left | ||
"></td> | "></td> | ||
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<td colspan="2" class="subHeader">Part Name:</td> | <td colspan="2" class="subHeader">Part Name:</td> | ||
- | <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with normal lacZ promoter </strong></td> | + | <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with normal lacZ promoter </strong></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader">Part Name:</td> | <td colspan="2" class="subHeader">Part Name:</td> | ||
- | <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with mutated lacZ promoter </strong></td> | + | <td colspan="2" class="bodyText"><strong>Upper primer for <em>aiiA</em> with mutated lacZ promoter </strong></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add a mutated version of lacZ promoter to <em>aiiA</em> in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add cI promoter upstream of the RcnR operator in the device cI | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA </p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" class="bodyText"><p>Lower primer for RcnA (BBa_K119003). Includes | + | <td colspan="2" class="bodyText"><p align="justify">Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region.</p></td> |
</tr> | </tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td colspan="3" class="calHeader">Devices</td> | + | <td colspan="3" class="calHeader style2"><a name="devices"></a>Devices</td> |
<td class="calHeader"> </td> | <td class="calHeader"> </td> | ||
</tr> | </tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion. </p></td> | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion. </p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader">Designed by:</td> | <td colspan="2" class="subHeader">Designed by:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td> | + | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em> expression. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA.</em></p></td> |
</tr> | </tr> | ||
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<td colspan="2" class="subHeader">Designed by:</td> | <td colspan="2" class="subHeader">Designed by:</td> | ||
- | <td colspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</td> | + | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader" >Description:</td> | <td colspan="2" class="subHeader" >Description:</td> | ||
- | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes | + | <td colspan="2" rowspan="2" class="bodyText"><p align="justify">This device encodes CI, the protein which controls <em>rcnA</em>. It also contains <em>luxR</em>, which induces the expression of <em>cI</em> and <em>aiiA</em> </p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="subHeader">Designed by:</td> | <td colspan="2" class="subHeader">Designed by:</td> | ||
- | <td colspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</td> | + | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td colspan="2" class="calHeader"> </td> | <td colspan="2" class="calHeader"> </td> | ||
</tr> </table> | </tr> </table> | ||
- | <br /> | + | <div align="center"><br /> |
- | + | <a href="#regparts"><img src="https://static.igem.org/mediawiki/2008/1/1f/Regparts1.jpg" alt="Regulatory parts" width="190" height="31" border="0"></a> <a href="#codparts"><img src="https://static.igem.org/mediawiki/2008/2/25/Regparts2.jpg" alt="Coding parts" width="190" height="31" border="0"></a><a href="#primers"><img src="https://static.igem.org/mediawiki/2008/8/8b/Regparts3.jpg" alt="Primers" width="190" height="31" border="0"></a><a href="#devices"><img src="https://static.igem.org/mediawiki/2008/d/d9/Regparts4.jpg" alt="Devices" width="190" height="31" border="0"></a><br> | |
+ | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /><br /> | ||
+ | <br /> | ||
+ | <br /> | ||
+ | </div> | ||
<td width="132"> </td> | <td width="132"> </td> | ||
</tr> | </tr> |
Revision as of 18:43, 28 October 2008
LCG-UNAM-Mexico | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
iGEM 2008 TEAM | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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