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Team:BrownTwo/Implementation/testing
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Apoptosis test- induce apoptosis externally and then save cells with the limiter device! | Apoptosis test- induce apoptosis externally and then save cells with the limiter device! | ||
| + | -this test has important medical relevance, seeing as many tumor phenotypes are characterized by a noted decline or absence of apoptotic activity | ||
| + | |||
| + | -for yeast, extrinsically-signaled apoptosis depends on oxidative stress or salt | ||
| + | -involves the following system: | ||
| + | Possible target for downregulation could be Bir1p (from BIR1 gene), a homolog of survivin from humans that is thought to stabilize XIAP | ||
| + | Another possible target is AIF1, a homolog of the human AIF1 | ||
| + | -released from mito.and travels to nucleus | ||
| + | -interesting fact about S. cerevisiae is that they are facultative anaerobic yeast and can survive even with complete removal of mitochondria, making them an ideal system for studying PCD (Frolich- Yeast apoptosos) | ||
| + | |||
| + | http://www.nature.com/embor/journal/v6/n11/full/7400514.html | ||
Revision as of 07:01, 28 October 2008
Circuit TestingInputs
Outputs
Viability TestsApoptosis test- induce apoptosis externally and then save cells with the limiter device! -this test has important medical relevance, seeing as many tumor phenotypes are characterized by a noted decline or absence of apoptotic activity -for yeast, extrinsically-signaled apoptosis depends on oxidative stress or salt -involves the following system: Possible target for downregulation could be Bir1p (from BIR1 gene), a homolog of survivin from humans that is thought to stabilize XIAP Another possible target is AIF1, a homolog of the human AIF1 -released from mito.and travels to nucleus -interesting fact about S. cerevisiae is that they are facultative anaerobic yeast and can survive even with complete removal of mitochondria, making them an ideal system for studying PCD (Frolich- Yeast apoptosos) http://www.nature.com/embor/journal/v6/n11/full/7400514.html |
