Team:Heidelberg/Notebook/Visualization/Notebook/1stweek

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[[Team:Heidelberg/Notebook/Visualization/Notebook|Overview]]<br>
[[Team:Heidelberg/Notebook/Visualization/Notebook|Overview]]<br>

Revision as of 07:57, 28 October 2008

Overview

Contents

First week

Monday 01/09/2008

  • Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.

First microscope test using MG1655 in 6 small chambers from Labtek:

3 types each type twice:
I - 0.1% agar in LB
II - 0.25% agar in LB
III - 0.5% agar in LB

View:

I II III
I II III

  • In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
  • Because the III. solution was more viscous 150 µl were necessary to cover the ground.
  • The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) They have been set under the media or in between.
  • About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
  • The chambers were incubated overnight at 37 °C. For microscope on the next day.

Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center.
-> In the further future 150 - 200 µl will be used.

Tuesday 02/09/2008

Wednesday 03/09/2008

Thursday 04/09/2008

Friday 05/09/2008

Saturday 06/09/2008

Sunday 07/09/2008

2ndWeek

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