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Team:Princeton/Experiments
From 2008.igem.org
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1. Primer Design | 1. Primer Design | ||
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2. PCR | 2. PCR | ||
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3. Run Gel | 3. Run Gel | ||
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4. Gel Extraction | 4. Gel Extraction | ||
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5. Digestion | 5. Digestion | ||
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6. CIP Treatment | 6. CIP Treatment | ||
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7. PCR Purification | 7. PCR Purification | ||
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8. Ligation | 8. Ligation | ||
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9. Transformation and Plating | 9. Transformation and Plating | ||
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10. Extract DNA (Miniprep) | 10. Extract DNA (Miniprep) | ||
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11. Restriction Map/ Digest | 11. Restriction Map/ Digest | ||
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12. Re-transform with selected plasmid | 12. Re-transform with selected plasmid | ||
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13. Extract DNA (Maxiprep or Midiprep) | 13. Extract DNA (Maxiprep or Midiprep) | ||
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14. Sequence | 14. Sequence | ||
Cell Culture | Cell Culture | ||
Revision as of 08:25, 28 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
|---|
| Parts Submitted to the Registry | Modeling | The Team | Gallery |
|---|
Bio-brick Creation
Lab Protocols
1. Primer Design
2. PCR
3. Run Gel
4. Gel Extraction
5. Digestion
6. CIP Treatment
7. PCR Purification
8. Ligation
9. Transformation and Plating
10. Extract DNA (Miniprep)
11. Restriction Map/ Digest
12. Re-transform with selected plasmid
13. Extract DNA (Maxiprep or Midiprep)
14. Sequence
Cell Culture
