Team:Princeton/Experiments

From 2008.igem.org

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1. Primer Design
1. Primer Design
-
2. PCR
+
2. PCR Amplification
-
3. Run Gel
+
3. PCR SOEing
-
4. Gel Extraction
+
4. Run Gel
-
5. Digestion
+
5. Gel Extraction
-
6. CIP Treatment
+
6. Digestion
-
7. PCR Purification
+
8. CIP Treatment
-
8. Ligation
+
8. PCR Purification
-
9. Transformation and Plating
+
9. Ligation
-
10. Extract DNA (Miniprep)
+
10. Transformation and Plating
-
11. Restriction Map/ Digest
+
11. Extract DNA (Miniprep)
-
12. Re-transform with selected plasmid
+
12. Restriction Map/ Digest
-
13. Extract DNA (Maxiprep or Midiprep)
+
13. Re-transform with selected plasmid
-
14. Sequence
+
14. Extract DNA (Maxiprep or Midiprep)
 +
 
 +
15. Sequence
===Bio-Brick Verification===
===Bio-Brick Verification===

Revision as of 17:01, 28 October 2008

PrincetonLogo.gif

PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. PCR SOEing

4. Run Gel

5. Gel Extraction

6. Digestion

8. CIP Treatment

8. PCR Purification

9. Ligation

10. Transformation and Plating

11. Extract DNA (Miniprep)

12. Restriction Map/ Digest

13. Re-transform with selected plasmid

14. Extract DNA (Maxiprep or Midiprep)

15. Sequence

Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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