Team:Edinburgh/Results

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Revision as of 16:24, 28 October 2008

Results and current status as of 29 October 2008. We are hoping to obtain some last-minute results to present at the Jamboree.

Overview

Our results are promising but our dream of using bacteria to convert cellulose into starch and β-carotene is still unrealised. Fortunately, the work will be continued by two undergraduate students after Christmas.

Cellulolysis device

cenA (endoglucanase) and cex (exoglucanase): Biobricks made and ribosome binding site added; ready to test.
bglX (beta glucosidase): Biobrick made and ribosome binding site added; ready to test.
P_cstA (promoter): Biobrick made and tested - successful.
- P_cstA promoter assay with xylE as repoter

Glycogenesis device

glgC and glgC16 (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.

Starch synthesis device

su1 and iso2 (isoamylases): Work in progress - pre-BioBrick constructs in pSB1A2 have been made using the BABEL protocol, but it is still necessary to remove internal EcoRI sites before fully compliant BioBricks will be ready to test. This work will be continued in an undergraduate honours project.

Lycopene generator

dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.

Limonene synthase device

dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).

@@ Line 38: / Line 38: @@
 * [[Team:Edinburgh/Results/BABEL|BABEL: BioBrick assembly with blunt-ended ligation]]
 * [[Team:Edinburgh/Results/Bacillobricks|Bacillobricks: introduction of BioBricks into ''Bacillus subtilis'']]
-==Acknowledgements==
-We would like to thank the following researchers for their contributions to our project:
-* Raman spectroscopy for glycogen production was done by '''Dr Rabah Mouras'''.
-* Mutagenesis of ''crtI'' was done by '''Douglas Armstrong'''.
-* The maize isoamylase genes ''su1'' and ''iso2'' were generously donated by '''Professor Alan Myers''' of Iowa State University.