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Team:Princeton/Experiments
From 2008.igem.org
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1. [[Primer Design]] | 1. [[Primer Design]] | ||
| - | 2. PCR Amplification | + | 2. [[PCR Amplification]] |
| - | 3. PCR SOEing | + | 3. [[PCR SOEing]] |
| - | 4. Run Gel | + | 4. [[Run Gel]] |
| - | 5. Gel Extraction | + | 5. [[Gel Extraction]] |
| - | 6. Digestion | + | 6. [[Digestion]] |
| - | 8. CIP Treatment | + | 8. [[CIP Treatment]] |
| - | + | 9. [[PCR Purification]] | |
| - | + | 10. [[Ligation]] | |
| - | + | 11. [[Transformation and Plating]] | |
| - | + | 12. [[Extract DNA (Miniprep)]] | |
| - | + | 13. [[Restriction Map/ Digest]] | |
| - | + | 14. [[Re-transform with selected plasmid]] | |
| - | + | 15. [[Extract DNA (Maxiprep or Midiprep)]] | |
| - | + | 16. [[Sequence]] | |
===Bio-Brick Verification=== | ===Bio-Brick Verification=== | ||
Revision as of 17:09, 28 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
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| Parts Submitted to the Registry | Modeling | The Team | Gallery |
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Bio-brick Creation
Lab Protocols
3. PCR SOEing
4. Run Gel
6. Digestion
10. Ligation
11. Transformation and Plating
14. Re-transform with selected plasmid
15. Extract DNA (Maxiprep or Midiprep)
16. Sequence
Bio-Brick Verification
Cell Culture Process
1. Lentivirus Production
2. Lentivirus Harvesting
3. Infection
Also,
1. Transfection (nonviral)
