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Team:Hawaii/Protocols
From 2008.igem.org
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== List of Protocols == | == List of Protocols == | ||
This is a list of tried and true protocols used by our team. | This is a list of tried and true protocols used by our team. | ||
| - | + | ===''E. coli''=== | |
* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | * [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | ||
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | ||
| - | |||
* Alkaline lysis / plasmid isolation | * Alkaline lysis / plasmid isolation | ||
| + | === ''Synechocystis'' sp. PCC6803=== | ||
| + | * [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]] | ||
| + | |||
| + | === Media=== | ||
* [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]] | * [[Team:Hawaii/Protocols/Solid_growth_media|Solid growth media]] | ||
:* LB | :* LB | ||
| Line 19: | Line 22: | ||
:* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub> | :* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub> | ||
* Liquid growth media | * Liquid growth media | ||
| + | |||
| + | === BioBrick === | ||
| + | * [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial] | ||
| + | * [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy] | ||
| + | * [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers] | ||
| + | * [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication] | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 22:44, 24 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
E. coli
- Making competent cells
- Transformation of E. coli
- Alkaline lysis / plasmid isolation
Synechocystis sp. PCC6803
Media
- LB
- LB + amp100
- LB + kan20 + amp 50
- LB + sp100
- LB + sp100 + sm50
- BG-11 + 5% LB
- BG-11
- BG-11 + sp2.5 + sm2.5
- Liquid growth media
BioBrick
- [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
- [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
- [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
