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Team:BCCS-Bristol/Parts
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[http://partsregistry.org/Part:BBa_K135000 BBa_K135000] | [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] | ||
| - | This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which | + | This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore adhesion sensitive. |
Revision as of 22:34, 28 October 2008
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Submitted Bio-Bricks
[http://partsregistry.org/Part:BBa_K135000 BBa_K135000]
This BioBrick contains pCpxR, a promoter which is responsive to phosphorylated CpxR (CpxR-P). Upon adhesion to hydrophobic surfaces, the CpxRA two-component system is activated. The inner membrane histidine kinase CpxA phosphorylates CpxR which then becomes active and regulates a number of target genes. This promoter is therefore adhesion sensitive.
[http://partsregistry.org/Part:BBa_K135001 BBa_K135001]
In this part, the the CpxR-P responsive promoter pCpxR is fused to the BioBrick [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] which encodes GFP. Thus, GFP is expressed after binding to hydrophobic surfaces (see also [http://partsregistry.org/Part:BBa_K135000 BBa_K135000] for further explanations).
