Team:University of Washington

From 2008.igem.org

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Revision as of 17:58, 27 June 2008

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.

Hi, to all.

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Our project seeks to control transfer of genetic material across phylogenetic kingdoms. Although the design is intended to promote modular substituion for use across different organisms and under various conditions, we will specifically attempt to transfer conjugative plasmids from the prokaryote Escherichia coli (kingdom Bacteria) to the yeast Saccharomyces cervisiae (kingdom Fungi). Essentially, the conjugative bacteria will only express conjugative machinery and transfer genetic material in the presence of certain species, the absence of others, and when yeast is present.

The conjugative plasmids transferred from E. coli to S. cervisiae will confer the ability for yeast to perform some adaptive function; in our case, it will be the ability to digest lactose. We will induce the production of conjugation machinery in E. coli under conditions where lactose is prevalent, glucose is minimal, and yeast proximity is sensed via a yeast-produced signaling molecule. The use of mutualistic interactions under conditions of selective pressure will assist in maintaining long-term functionality of genetic circuitry. Future directions of this project might include clinical gene delivery, more reliable transformation of other eukartyoes, and other interacting multiple-organism systems.

Your team picture

Team Example 2

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

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 ''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+Our project seeks to control transfer of genetic material across phylogenetic kingdoms. Although the design is intended to promote modular substituion for use across different organisms and under various conditions, we will specifically attempt to transfer conjugative plasmids from the prokaryote ''Escherichia coli'' (kingdom Bacteria) to the yeast ''Saccharomyces cervisiae'' (kingdom Fungi). Essentially, the conjugative bacteria will only express conjugative machinery and transfer genetic material in the presence of certain species, the absence of others, and when yeast is present.
+The conjugative plasmids transferred from ''E. coli'' to ''S. cervisiae'' will confer the ability for yeast to perform some adaptive function; in our case, it will be the ability to digest lactose. We will induce the production of conjugation machinery in ''E. coli'' under conditions where lactose is prevalent, glucose is minimal, and yeast proximity is sensed via a yeast-produced signaling molecule. The use of mutualistic interactions under conditions of selective pressure will assist in maintaining long-term functionality of genetic circuitry. Future directions of this project might include clinical gene delivery, more reliable transformation of other eukartyoes, and other interacting multiple-organism systems.
 |[[Image:Team.png|right|frame|Your team picture]]
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