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Team:The University of Alberta/21 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: i digested: pUC57-pTET-BisdA with SphI+PstI with CIP treatment pTET-BisdB with XbaI+PstI laqIQ-ERE-TetR with XbaI+PstI i set up the following ligations with the gel purified prod...) |
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pUC57-pTET-BisdA with SphI+PstI with CIP treatment | pUC57-pTET-BisdA with SphI+PstI with CIP treatment | ||
Revision as of 03:05, 29 October 2008
David
i digested:
pUC57-pTET-BisdA with SphI+PstI with CIP treatment pTET-BisdB with XbaI+PstI laqIQ-ERE-TetR with XbaI+PstI
i set up the following ligations with the gel purified products:
pUC57-pTET-BisdA+pTET-BisdB pSB103+laqIQ-ERE-TetR
overnight at 16 degrees C.
