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Team:USTC/Results
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=== Correlation between GFP generation and cell density === | === Correlation between GFP generation and cell density === | ||
==Function Test == | ==Function Test == | ||
| + | After GFP(LVA) cloned successfully,we want to test its function.We ligate it with lac promoter R0010,a strong RBS B0034 and teminator B0015. We transform the plasmid with these parts to E.coli strain TOP10 and induce the GFP express with 0.1mM IPTG. | ||
:::::[[Image:USTC-result1.jpg|left|300px|thumb|fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.]] | :::::[[Image:USTC-result1.jpg|left|300px|thumb|fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.]] | ||
:::::::::::::::::::[[Image:USTC-result2.jpg|left|300px|thumb|fig 8.Cells that generate GFP in fluorescence microscope ]] | :::::::::::::::::::[[Image:USTC-result2.jpg|left|300px|thumb|fig 8.Cells that generate GFP in fluorescence microscope ]] | ||
Revision as of 09:41, 29 October 2008
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Correlation between GFP generation and cell density
Function Test
After GFP(LVA) cloned successfully,we want to test its function.We ligate it with lac promoter R0010,a strong RBS B0034 and teminator B0015. We transform the plasmid with these parts to E.coli strain TOP10 and induce the GFP express with 0.1mM IPTG.
fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.
fig 8.Cells that generate GFP in fluorescence microscope
