"
Team:Edinburgh/Plan/Beta-Carotene
From 2008.igem.org
(Difference between revisions)
(→β-carotene synthesis) |
(→Directly involved genes from P. ananatis) |
||
| Line 11: | Line 11: | ||
# Lycopene is produced from phytoene by phytoene desaturase ([https://2008.igem.org/Team:Edinburgh/crtI '''''crtI''''']). | # Lycopene is produced from phytoene by phytoene desaturase ([https://2008.igem.org/Team:Edinburgh/crtI '''''crtI''''']). | ||
# Finally, lycopene β-cyclase ([https://2008.igem.org/Team:Edinburgh/crtY '''''crtY''''']) cyclises lycopene to produce β-carotene. | # Finally, lycopene β-cyclase ([https://2008.igem.org/Team:Edinburgh/crtY '''''crtY''''']) cyclises lycopene to produce β-carotene. | ||
| + | |||
| + | See figure 1 for structure details. | ||
== Indirectly involved genes from ''E. coli'' == | == Indirectly involved genes from ''E. coli'' == | ||
Revision as of 16:18, 29 October 2008
Contents |
β-carotene synthesis
β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering carotenoid synthesis genes from Pantoea ananatis, a member of the proteobacteria naturally capable of producing β-carotene. The second involves upregulating the glycolysis pathways in E. coli in order to concentrate more energy into making β-carotene.
Directly involved genes from P. ananatis
- Geranyl diphosphate synthase (crtE) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.
- Geranyl geranyl diphosphate is then converted into phytoene by phytoene synthase (crtB).
- Lycopene is produced from phytoene by phytoene desaturase (crtI).
- Finally, lycopene β-cyclase (crtY) cyclises lycopene to produce β-carotene.
See figure 1 for structure details.
Indirectly involved genes from E. coli
Overview
- In blue are the genes which we manipulated.
