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Team:LCG-UNAM-Mexico/Experiments/Experiments
From 2008.igem.org
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<p>Once the electronic device was built, We investigated the range where it was able to measure nickel concentrations on LB medium. We found that the device has maximum efficiency in the range (1e-7,4e-4).</p> | <p>Once the electronic device was built, We investigated the range where it was able to measure nickel concentrations on LB medium. We found that the device has maximum efficiency in the range (1e-7,4e-4).</p> | ||
| - | <p><img src="https:// | + | <p align="justify"><img src="https://static.igem.org/mediawiki/2008/b/bf/Ni_Res_loglogLR.jpg" width="595" height="350"></p> |
| + | <p align="justify">The graph shows that increasing the nickel level decreases resistivity which is in accordance with the ionic nature of nickel. Points represent the mean of three independent replicates and the line represents the linear regression model:</p> | ||
| + | <p align="justify"><img align="left" src="https://static.igem.org/mediawiki/2008/a/a7/LR_Ni_re.png" width="230" height="30"><br><br></p> | ||
| + | <p align="justify">Where Ω is the resistivity measured in ohms and [Ni] is the nickel concentration.This shows that even in a complex medium (LB) we are able to detect changes in nickel concentration in a wide range that spans several order of magnitude.</p> | ||
| + | <p align="justify">Then we investigated the time dynamics of resistivity in the presence of cells in the previously defined range of nickel concentrations. For this purpose we used cells lacking the rcnA gene, to reduce the effect of cells on our measurements. We have to take into account that even these cells add a variable to our measurements because they continuously import nickel to their cytoplasm.</p> | ||
| + | <p align="justify">All our measurements lasted three minutes and we have one data point each 10ms, which means that we have 18000 data points for each experiment. The plot below shows means of each time point for two or three replicates at the indicated nickel concentration. The blue line represents experiments where nickel was not added.</p> | ||
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Revision as of 16:39, 29 October 2008
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Once the electronic device was built, We investigated the range where it was able to measure nickel concentrations on LB medium. We found that the device has maximum efficiency in the range (1e-7,4e-4).
The graph shows that increasing the nickel level decreases resistivity which is in accordance with the ionic nature of nickel. Points represent the mean of three independent replicates and the line represents the linear regression model:
Where Ω is the resistivity measured in ohms and [Ni] is the nickel concentration.This shows that even in a complex medium (LB) we are able to detect changes in nickel concentration in a wide range that spans several order of magnitude. Then we investigated the time dynamics of resistivity in the presence of cells in the previously defined range of nickel concentrations. For this purpose we used cells lacking the rcnA gene, to reduce the effect of cells on our measurements. We have to take into account that even these cells add a variable to our measurements because they continuously import nickel to their cytoplasm. All our measurements lasted three minutes and we have one data point each 10ms, which means that we have 18000 data points for each experiment. The plot below shows means of each time point for two or three replicates at the indicated nickel concentration. The blue line represents experiments where nickel was not added.
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