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Team:Heidelberg/Notebook/Visualization/Notebook/SwarmAssays
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Petri-dishes were used es running chambers.<br> | Petri-dishes were used es running chambers.<br> | ||
| - | A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours. | + | A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.<br> |
| - | For a more detailed protocol visit the [https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/ | + | For a more detailed protocol visit the [https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Methods methods] page.<br> |
Revision as of 16:18, 29 October 2008
Swarm Assays
STARTED 2008-09-27
PURPOSE:
Test of the abbility of strains expressing the fusion receptor, to move against an attractant gradient.
STRAIN:
As autoinducer 2 producing attactant strain DH5α was used.
The fusion receptor plasmid was further cloned into HCB33 and UU1250 strains.
METHOD:
Petri-dishes were used es running chambers.
A petri-dish was filled with a media containig agar. Then the attractant was spotted on premarked positions or spotted as a line through the center of the petri-dish. After the gradient of the attractant has etablished the strains whioch should be attracted by the attractant where also set on premarked positions. The whole petri-dish was incubated at 37 °C and pictures were taken after 24, 48 and 72 hours.
For a more detailed protocol visit the methods page.
A few pictures which have been taken during these experiments can be viewed under Projects.
back to the visualization notebook
