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Alberta NINT/24 June 2008
From 2008.igem.org
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From results it seems ligation didn't work. | From results it seems ligation didn't work. | ||
Quantified K102003/B+N. | Quantified K102003/B+N. | ||
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[[Alberta_NINT/25_June_2008 | Next entry >]] | [[Alberta_NINT/25_June_2008 | Next entry >]] | ||
Revision as of 00:24, 15 July 2008
lab work (SD):
Transformation results: 2 colonies on K102008 plate and 2 colonies on K102008 negative control plate
Redid ligation of K102002/B+N with TA0/B+N (1/100) and transformation into XL1-B cells.
Made overnights of the colonies from 23/06/08 transformation.
Made glycerol stock of K102002.
lab work (JD):
Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C
Ran gel slowly on 2% agarose
Cut out desired fragment and gel purified
Counted and recorded colonies from yesterdays ligation:
Negative 200
K102007 200
From results it seems ligation didn't work.
Quantified K102003/B+N.
