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Team:Chiba/Calendar-Home/20 August 2008
From 2008.igem.org
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<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>100μL</td><td>100μL</td><td>100μL</td><td>100μL</td><td>100μL</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 72: | Line 72: | ||
<tr> | <tr> | ||
<td>Total</td> | <td>Total</td> | ||
| - | <td> | + | <td>6μL</td><td>6μL</td><td>6μL</td><td>6μL</td><td>6μL</td> |
</tr> | </tr> | ||
Revision as of 19:50, 29 October 2008
19 August 2008 <|> 21 August 2008
Contents |
Laboratory work
Team:Input
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
- [http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)
| Sample No. | 1 | 2 | 3 | 4 | 5 |
| DNA tamplate | 1 | 1 | 1 | 1 | >1 |
| FW primer | 5 | 5 | 5 | 5 | 5 |
| VR primer | 5 | 5 | 5 | 5 | 5 |
| dNTPmix | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 |
| dH2O | 68 | 68 | 68 | 68 | 68 |
| TOTAL | 100μL | 100μL | 100μL | 100μL | 100μL |
-->Gel Check
| Sample No. | 1 | 2 | 3 | 4 | 5 |
| Sample DNA | 1 | 1 | 1 | 1 | >1 |
| loading dye | 1 | 1 | 1 | 1 | 1 |
| dH2O | 4 | 4 | 4 | 4 | 4 |
| Total | 6μL | 6μL | 6μL | 6μL | 6μL |
-->1,2,3,4,5:none
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
- [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
- [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
- [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony
Team:Communication
- (18/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
- [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
- --->Gel Check
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--->Digestion test
- [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)
| Sample No | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) | 3 | - | - | - | 3 | - | - | - |
| [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) | - | 3 | - | - | - | 3 | - | - |
| [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) | - | - | 3 | - | - | - | 3 | - |
| [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) | - | - | - | 3 | - | - | - | 3 |
| EcoRⅠ | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| PstⅠ | - | - | - | - | 0.1 | 0.1 | 0.1 | 0.1 |
| Buffer EcoRⅠ | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| BSA | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O | 4.9 | 4.9 | 4.9 | 4.9 | 4.8 | 4.8 | 4.8 | 4.8 |
| TOTAL | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
- --->Gel Check
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Team:Output
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
- [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
- [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
- [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
- YFP(P-GEX)-->colony(Amp)
- [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony
