Team:Chiba/Calendar-Home/10 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
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===Team:Communication===
===Team:Communication===
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(9/9)-->
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(9/9)-->'''Colony Count'''
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:Colony Count
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#LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
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#LuxI
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#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
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#LuxI with LVA
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#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
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#Las
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#Background(R0010)
#Background(R0010)
#Background(C0261)
#Background(C0261)
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'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
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::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620　 BBa_F2620](2007)).
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::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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</table>
</table>
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??
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:95&deg;C,5min -> ( 95&deg;C,1min -> 52&deg;C,1min -> 72&deg;C,1min )･･･25cycles -> 72&deg;C,10min -> 6&deg;C
:95&deg;C,5min -> ( 95&deg;C,1min -> 52&deg;C,1min -> 72&deg;C,1min )･･･25cycles -> 72&deg;C,10min -> 6&deg;C
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??
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-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''

Revision as of 19:40, 29 October 2008

>Home | Notebook

9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->Colony Count

  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background(R0010)
  5. Background(C0261)

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


-->Gel Check

--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

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