Team:Chiba/Calendar-Home/31 August 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Output)
 
Line 143: Line 143:
[[Team:Chiba/protocol/digestion|Digestion]]
[[Team:Chiba/protocol/digestion|Digestion]]
-
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
+
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
-
*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②
+
*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>②</td>
+
<td>1</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
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</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
<td>1</td><td>0</td>
<td>1</td><td>0</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ</td>
+
<td>XbaI</td>
<td>0</td><td>1</td>
<td>0</td><td>1</td>
</tr>
</tr>
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-->left for 3 hours at 37 °C.
-->left for 3 hours at 37 °C.
-
-->①20μl refine
+
-->(1)20μl refine
-
-->②20μl refine
+
-->(2)20μl refine

Latest revision as of 20:41, 29 October 2008

>Home | Notebook

30 August 2008 <|> 1 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
Competent Cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
---> We saved these plates with a refrigerator.


Digestion
  1. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  2. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  3. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1~34
Sample DNA(μL)1210
PstⅠ(μL) 0.20.2
XbaⅠ(μL)0.2-
SpeⅠ(μL)-0.4
Buffer 2(μL) -1.5
Buffer 3(μL) 2-
BSA(μL) 21.5
dH2O(μL) 3.61.4
TOTAL(μL) 2015


--->(1/9)Gel Check


(30/8)--->Mini prep


--->Digestion test
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
Sample Single Digesiton 2~5Double Digestion 2~5Single Digestion T9002(1)Single Digesiton T9002(2)Double Digestion T9002(1)(2)
Sample DNA(μL)13313
XbaI(μL)0.10.10.10.10.1
SpeI(μL)-0.1--0.1
Buffer 2(μL) 0.90.80.90.90.8
BSA(μL) 11111
dH2O(μL) 75575
TOTAL(μL) 1010101010


--->Gel Check
Chiba-0831-p87.JPG
Sample No. [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2) Single & Double Digestion
Sample DNA(μL) 110
Loading Dye(μL) 12
dH2O(μL) 4-
TOTAL(μL) 612
From Left
  • Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
  • Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Team:Output

Digestion

  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)
Sample No. 12
DNA 1010
SpeI 10
PstI 11
XbaI 01
BSA(x100) 0.50.5
Buffer2 50
Buffer3 05
dH2O 28.528.5
TOTAL 50μl50μl

-->left for 3 hours at 37 °C.

-->(1)20μl refine

-->(2)20μl refine

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