Team:Hawaii/Parts

From 2008.igem.org

(Difference between revisions)
(→Parts in process (building))
Line 35: Line 35:
! width="250"|Description
! width="250"|Description
! width="75"|Type
! width="75"|Type
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
 
-
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (rbs)
 
-
| plasmid
 
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125010 '''K125010''']
Line 47: Line 43:
| rep/oriv/aada/orit
| rep/oriv/aada/orit
| intermediate for RSF1010 derived plasmid
| intermediate for RSF1010 derived plasmid
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
 
-
| nitrate inducible ''nir'' promoter
 
-
| regulator
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
 
-
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
 
-
| regulator
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125300 '''K125300''']
 
-
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
 
-
| protein coding
 
-
|- style="background:#f0f0f0; color:black"
 
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 '''K125310''']
 
-
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
 
-
| protein coding
 
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125320 '''K125320''']
| oriT derived from RP4
| oriT derived from RP4
| origin of transfer
| origin of transfer
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125330 '''K125330''']
 +
| P1 lytic replication
 +
|
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125340 '''K125340''']
 +
| oriV
 +
|
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125350 '''K125350''']
Line 76: Line 64:
| conjugation screening
| conjugation screening
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125510 '''K125510''']
-
| GFP fusion protein
+
| Plasmid replication proteins
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
Line 92: Line 80:
| protein coding
| protein coding
|- style="background:#f0f0f0; color:black"
|- style="background:#f0f0f0; color:black"
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
+
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125830 '''K125830''']
-
| N-terminal slr2016 signal sequence attached to GFP fusion protein
+
| ''Synechocystis''-specific, nitrate-inducible GFP secretion device under the control of a ''nirA'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
-
| protein coding
+
-
|- style="background:#f0f0f0; color:black"
+
-
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
+
-
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter
+
| device
| device
|}
|}
==Parts submitted==
==Parts submitted==
 +
{| style="background:#BCEE68;color:green;" border="0" cellpadding="10" cellspacing="0" align="center"
 +
|-
 +
! width="75"|Part number
 +
! width="250"|Description
 +
! width="75"|Type
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125000 '''K125000''']
 +
| pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (''lac'' promoter+''lacZ'')
 +
| plasmid
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125100 '''K125100''']
 +
| nitrate inducible ''nir'' promoter
 +
| regulator
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125110 '''K125110''']
 +
| nitrate inducible ''nir'' promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030]
 +
| regulator
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125300 '''K125300''']
 +
| cyanobacterial pilA derived amino termninus protein secretion signal sequence
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 '''K125310''']
 +
| cyanobacterial slr2016 derived amino terminus protein secretion signal sequence
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125500 '''K125500''']
 +
| GFP fusion protein
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125810 '''K125810''']
 +
| N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| protein coding
 +
|- style="background:#f0f0f0; color:black"
 +
| [http://partsregistry.org/wiki/index.php/Part:BBa_K125820 '''K125820''']
 +
| ''Synechocystis''-specific constitutive GFP secretion device under the control of a ''lac'' promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500])
 +
| device
 +
|}

Revision as of 22:17, 29 October 2008

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] Broad-Host-Range plasmid derived from RSF1010 plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] oriT derived from RP4 origin of transfer
[http://partsregistry.org/wiki/index.php/Part:BBa_K125330 K125330] P1 lytic replication
[http://partsregistry.org/wiki/index.php/Part:BBa_K125340 K125340] oriV
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] oriTr/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] oriT/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125510 K125510] Plasmid replication proteins protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] aadA gene protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] aadA gene with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] rep proteins with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125830 K125830] Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device

Parts submitted

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] nitrate inducible nir promoter regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device
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