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| - | >[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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| - | [[Team:Chiba/Calendar-Home/8 September 2008|8 September 2008 <]]|[[Team:Chiba/Calendar-Home/10 September 2008|> 10 September 2008]]
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| - | ==Laboratory work==
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| - | ===Team:Input===
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| - | no work
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| - |
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| - | ===Team:Communication===
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| - | (6/9)--->[[Team:Chiba/protocol/PCR|PCR]] of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]
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| - |
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| - |
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| - |
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| - | --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
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| - | {|align="justify"
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| - | |[[Image:Chiba-0909-3.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>60</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>12</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>72μL</td>
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| - | </tr>
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| - | </table>
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| - | ::CinI
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| - | |}
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| - |
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| - |
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| - | --->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
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| - | ::*CinI
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| - | ::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
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| - | ::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
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| - | ::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
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| - | ::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
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| - |
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| - |
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| - | --->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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| - | {|align="justify"
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| - | |[[Image:Chiba-0909.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH<sub>2</sub>O</td>
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| - | <td>4</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>6μL</td>
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| - | </tr>
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| - | </table>
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| - | :From left;
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| - | ::CinI -> ?
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| - | ::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
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| - | ::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
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| - | ::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
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| - | ::Marker
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| - | ::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
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| - | |}
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| - |
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| - | {|align="justify"
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| - | |[[Image:Chiba-0909-2.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH<sub>2</sub>O</td>
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| - | <td>4</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>6μL</td>
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| - | </tr>
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| - | </table>
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| - | ::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
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| - | |}
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| - |
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| - | #LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
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| - | #LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
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| - | #LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
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| - | #Background([http://partsregistry.org/Part:BBa_R0010 R0010])
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| - | #Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
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| - |
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| - | :--->'''[[Team:Chiba/protocol/ligation/ligation|Ligation]]'''
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| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample No</td>
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| - | <td>(1)</td><td>(2)</td><td>(3)</td><td>(4)</td><td>(5)</td>
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| - | </tr>
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| - | <tr>
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| - | <td>insert-C0161</td>
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| - | <td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>insert-C0261</td>
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| - | <td>-</td><td>2</td><td>-</td><td>-</td><td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>insert-S03154</td>
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| - | <td>-</td><td>-</td><td>6</td><td>-</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Vector-R0010</td>
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| - | <td>-</td><td>5</td><td>5</td><td>5</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>vector-J04500</td>
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| - | <td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>ligase</td>
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| - | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Buffer</td>
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| - | <td>2</td><td>2</td><td>4</td><td>2</td><td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH<sub>2</sub>O</td>
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| - | <td>1</td><td>-</td><td>4</td><td>2</td><td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>10</td><td>10</td><td>20</td><td>10</td><td>10</td>
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| - | </tr>
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| - |
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| - |
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| - | :---> '''[[Team:Chiba/protocol/transformation|Transformation]]'''
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| - |
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| - | ---> '''Colony Count'''( 10/9)
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| - |
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| - | ===Team:Output===
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| - |
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| - | no work
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"
