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| - | == Applying bacterioclock to metabolic engineering of polyhydroxyalkanoate biosynthesis pathways ==
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| - | Human overpopulation combined with the current lifestyle urges the rational, efficient, and sustainable use of natural resources to produce environmentally friendly plastic materials.
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| - | One illustrative example is polyhydroxyalkanoic acids (PHAs), whose production/degradation cycle reduces undesirable wastes and emissions.
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| - | The biosynthesis of this polymer is currently subject to intensive work.
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| - | It consists in expressing in appropriate quantities of 3 enzymes PhaA ,PhaB and PhaC that sequentialy process AcetylcoA into its final product PHA.
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| - | This biosynthesis is subjected to two contraints :
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| - | * Intermediate products of this pathway are used in alternatives competing metabolic pathways,
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| - | * Only the final product is of interest,so that all intermediates products need to be transformed into PHA.
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| - | [[Image:PHA.jpg|center]]
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| - | two strategies are commonly used in bioengineering of methabolics pathways :
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| - | * Sequential expression of enzymes involved in the pathways,
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| - | * Or constitutive expressions of all enzymes.
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| - | Because of the above mentioned limitations, none of these approches are adapted here .
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| - | Using the sequential expression, intermediate products would accumulate and thus be consummed by competing pathways.
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| - | Using the constitutive expression a mixture of final and intermediate product would necessarily be obtained.
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| - | Our FIFO could be useful here.
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| - | Indeed a FIFO expression pattern is intermediate between a purely sequential and a purely constitutive expression.
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| - | At some point all enzymes are presents ( no accumulation of intermediate products ) and during the last step only the last enzyme (PhC) is presents ( all intermediate products are consummed).
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| - | Moreover, the fact that our system oscillate could provide to the cell a metabolic recovering phase.
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| | == More general applications == | | == More general applications == |
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