Team:Hawaii/Notebook/2008-10-26

From 2008.igem.org

(Difference between revisions)
(→Things we did today)
(→Wetlab work)
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:<strong> Margaret</strong>
:<strong> Margaret</strong>
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===Construction of BB-pRL1383a/K125820===
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===Construction of BB-pRL1383a/K125810===
:<strong>Krystle</strong>
:<strong>Krystle</strong>
:* Transformation of overnight ligation products
:* Transformation of overnight ligation products

Revision as of 01:18, 30 October 2008

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Things we did today

Wetlab work

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.
Grace
  • Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
  • Ran on gel to verify plasmid
  • ~2ng/μl
  • The prep kit yields really clean preps in ~1 hour. :D!

Plasmid prep

EtBr stained 0.8% agarose gel ran at 95V for 1 hour. 5 microliters of plasmid were loaded into wells 3 and 4 while ligation products were loaded into lanes 5 and 6.
Margaret

Construction of BB-pRL1383a/K125810

Krystle
  • Transformation of overnight ligation products
  • Plated onto LB sp100 plates

Drylab Work

Inputted parts in the registry

Krystle

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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