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Team:Hawaii/Notebook/2008-10-28
From 2008.igem.org
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:*Inoculated 10ml cultures of LB with | :*Inoculated 10ml cultures of LB with | ||
:** BB-pRL1383a with sp<sub>100</sub> | :** BB-pRL1383a with sp<sub>100</sub> | ||
| - | :** BB-pRL1383a/ | + | :** BB-pRL1383a/K125810 with sp<sub>100</sub> |
:** RP1 with kan<sub>50</sub> | :** RP1 with kan<sub>50</sub> | ||
Revision as of 01:17, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic test
- Grace
- Ran HindIII/BamHI digested J33207 on gel
- Extracted from gel
- Ligated with HindIII/BamHI digested pRL1383a
- Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
- Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Checked for Transformants
- Krystle
- BB-pRL1383a/K125810 produced a total of 27 transformants of varying size after incubation for 20 hours at 37oC
Inoculate for Tri-Parental Conjugation
- Krystle
- Inoculated 10ml cultures of LB with
- BB-pRL1383a with sp100
- BB-pRL1383a/K125810 with sp100
- RP1 with kan50
- Inoculated 10ml cultures of LB with
Drylab Work
Project page
- Grace
- Wrote up Materials, Methods and Results for Project Part B on team project page.
Sequence analysis
- Grace
- Same G->C transversion in CAP binding site for BBpRL-1 sequence.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
