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Team:Edinburgh/SU1
From 2008.igem.org
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=== Primers === | === Primers === | ||
| - | '''Forward:''' gat gaattc gcggccgc t tctag atg gcg cag ctc ccc tg | + | '''Forward:''' gat gaattc gcggccgc t tctag atg gcg cag ctc ccc tg<br /> |
'''Reverse:''' gct actagt a tta tta gac atc agg gcg caa tac | '''Reverse:''' gct actagt a tta tta gac atc agg gcg caa tac | ||
Latest revision as of 01:18, 30 October 2008
Contents |
SU1
Amino Acid Sequence
MAQQLPCVSSPRPlLAvPAGRWRAGvRGRPNvAGlGRGRlSLHAAAARPv
AEAvQAEEDDDDDDEEvAEERFAlGGACRvLAGMPAPLGATALRGGVNFA
VYSSGASAASlCLFAPGDLKADRvTEEvPLDPlLNRTGNvWHvFIHGDQl
HGMLYGYRFDGvFAPERGQYYDvSNVvvDPYAKAvVSRGEYGvPAPGGSC
WPQMAGMIPLPYNKFDWQGDLPLGYHQKDLVIYEMHlRGFTKHNSSKTKH
PGTYIGAvSKLDHLKELGvNCIELMPCHEFNELEYFSSSSKMNFWGYSTI
NFFSPMARYSSSGIRDSGCGAINEFKAFVREAHKRGIEvIMDVVFNHTAE
GNEKGPILSFRGIDNSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIvD
ClRYWVTEMHVDGFRFDLASIlTRGCSLWDPVNvYGSPMEGDMITTGTPL
VAPPLIDMISNDPILGNVKLIAEAWDAGGLYQVGQFPHWNVWSEWNGKYR
DTvRQFIKGTDGFAGAFAECLCGSPQLYQAGGRKPWHSINFVCAHDGFTl
ADlVTYNSKYNlSNGEDNRDGENHNLSWNCGEEGEFASlSVRRLRKRQMR
NFFVCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEEQS
SDlYRFCRLMTKFRKECESLGLEDFPTSERlKWHGHQPGKPDWSEASRFV
AFTMKDETKGEIYvAFNTSHLPvVVGLPERSGFRWEPvvDTGKEAPYDFL
TDGLPDRAVTVYQFSHFLNSNLYPMLSYSSIILVlRPDV*
DNA Sequence
atggcgcagcagctcccctgcgtctcgtcgccgcgcccgctgctcgccgt 50
accgcgtcgtcgaggggacgcagagcagcggcgcgggcgacgagcggca
gcccgcgggccggtggcgcgccggcgtgcggggccggcccaatgtggcgg 100
cgggcgcccggccaccgcgcggccgcacgccccggccgggttacaccgcc
gactggggcgggggcggctgtctctccacgccgccgccgcgcggcccgtg 150
ctgaccccgcccccgccgacagagaggtgcggcggcggcgcgccgggcac
gccgaggcggtgcaggcggaggaggacgacgacgacgacgacgaggaggt 200
cggctccgccacgtccgcctcctcctgctgctgctgctgctgctcctcca
ggccgaggagaggttcgcgctgggcggcgcgtgccgggtgctcgcgggaa 250
ccggctcctctccaagcgcgacccgccgcgcacggcccacgagcgccctt
tgcccgcgccgctcggcgccaccgcgctccgcggcggtgtcaacttcgcc 300
acgggcgcggcgagccgcggtggcgcgaggcgccgccacagttgaagcgg
gtctactccagcggtgcctccgccgcgtcgctgtgcctcttcgctcccgg 350
cagatgaggtcgccacggaggcggcgcagcgacacggagaagcgagggcc
cgacctcaaggcggatagggtgaccgaggaggtgcccctcgatcccctgc 400
gctggagttccgcctatcccactggctcctccacggggagctaggggacg
tcaaccgaacgggaaacgtgtggcacgtgttcatccacggggaccagctg 450
agttggcttgccctttgcacaccgtgcacaagtaggtgcccctggtcgac
cacggcatgctctacggatacaggttcgatggcgtgttcgcccctgagcg 500
gtgccgtacgagatgcctatgtccaagctaccgcacaagcggggactcgc
cggacagtactacgatgtgtccaacgttgtggtggatccatacgctaagg 550
gcctgtcatgatgctacacaggttgcaacaccacctaggtatgcgattcc
cagtggtaagccgaggtgaatatggtgtgcctgcgcctggtggtagttgt 600
gtcaccattcggctccacttataccacacggacgcggaccaccatcaaca
tggcctcaaatggctggtatgatccctcttccctataataagtttgattg 650
accggagtttaccgaccatactagggagaagggatattattcaaactaac
gcaaggtgacctaccccttgggtaccatcagaaggaccttgtcatatatg 700
cgttccactggatggggaacccatggtagtcttcctggaacagtatatac
aaatgcatttgcgtggattcacaaagcacaactcaagcaagacaaaacac 750
tttacgtaaacgcacctaagtgtttcgtgttgagttcgttctgttttgtg
ccaggaacttacattggtgctgtgtcaaagcttgaccatctaaaggaact 800
ggtccttgaatgtaaccacgacacagtttcgaactggtagatttccttga
tggagtgaactgtatagagctaatgccctgccatgagttcaatgagctag 850
acctcacttgacatatctcgattacgggacggtactcaagttactcgatc
agtacttcagctcctcttcgaagatgaacttctggggatattccacaata 900
tcatgaagtcgaggagaagcttctacttgaagacccctataaggtgttat
aattttttctcaccaatggcaagatattcttcaagtggcataagagactc 950
ttaaaaaagagtggttaccgttctataagaagttcaccgtattctctgag
tggatgtggtgccataaatgaatttaaagcttttgtaagggaggcccaca 1000
acctacaccacggtatttacttaaatttcgaaaacattccctccgggtgt
aacggggaattgaggtgatcatggatgttgtcttcaatcatacagctgaa 1050
ttgccccttaactccactagtacctacaacagaagttagtatgtcgactt
ggtaatgagaaaggcccaatattatcctttagggggatagataatagtac 1100
ccattactctttccgggttataataggaaatccccctatctattatcatg
atactacatgcttgcacctaagggagagttttataattattctggttgtg 1150
tatgatgtacgaacgtggattccctctcaaaatattaataagaccaacac
gaaataccttcaattgtaatcatcctgtagtccgtgaatttatagtggat 1200
ctttatggaagttaacattagtaggacatcaggcacttaaatatcaccta
tgcttgagatactgggtaacagaaatgcatgttgatggttttcgttttga 1250
acgaactctatgacccattgtctttacgtacaactaccaaaagcaaaact
ccttgcatctatactgaccagaggatgcagtctatgggatccagttaatg 1300
ggaacgtagatatgactggtctcctacgtcagataccctaggtcaattac
tgtatggaagtccaatggaaggtgacatgattacgacagggacacctctt 1350
acataccttcaggttaccttccactgtactaatgctgtccctgtggagaa
gttgccccaccacttattgacatgattagcaatgacccaattcttggaaa 1400
caacggggtggtgaataactgtactaatcgttactgggttaagaaccttt
tgtcaagctcattgctgaagcatgggatgcaggaggtctctatcaagttg 1450
acagttcgagtaacgacttcgtaccctacgtcctccagagatagttcaac
gtcagtttcctcactggaacgtttggtcagagtggaatggaaagtatcgc 1500
cagtcaaaggagtgaccttgcaaaccagtctcaccttacctttcatagcg
gataccgtgcgtcagttcatcaaaggcacagatggatttgctggtgcttt 1550
ctatggcacgcagtcaagtagtttccgtgtctacctaaacgaccacgaaa
tgctgaatgcctatgtggaagtccacagttataccaggcaggggggagga 1600
acgacttacggatacaccttcaggtgtcaatatggtccgtcccccctcct
agccttggcacagtatcaactttgtatgtgcacacgatggatttacactg 1650
tcggaaccgtgtcatagttgaaacatacacgtgtgctacctaaatgtgac
gctgatttggtcacatacaatagcaagtacaacttgtcaaatggtgagga 1700
cgactaaaccagtgtatgttatcgttcatgttgaacagtttaccactcct
caacagagatggggaaaatcataatcttagctggaattgtggggaggaag 1750
gttgtctctaccccttttagtattagaatcgaccttaacacccctccttc
gagaatttgcaagtctgtcagtccgaagattaaggaagaggcaaatgcgc 1800
ctcttaaacgttcagacagtcaggcttctaattccttctccgtttacgcg
aatttctttgtttgtcttatggtttctcagggagttccaatgttctacat 1850
ttaaagaaacaaacagaataccaaagagtccctcaaggttacaagatgta
gggcgatgaatatggtcacacaaagggagggaacaacaatacgtactgcc 1900
cccgctacttataccagtgtgtttccctcccttgttgttatgcatgacgg
atgaccattatgtcaactatttccgttgggataagaaggaagaacaatcc 1950
tactggtaatacagttgataaaggcaaccctattcttccttcttgttagg
tctgatttgtacagattctgccgtctcatgaccaaattccgcaaggaatg 2000
agactaaacatgtctaagacggcagagtactggtttaaggcgttccttac
tgaatctcttggccttgaggacttcccgacttcagaacggttgaaatggc 2050
acttagagaaccggaactcctgaagggctgaagtcttgccaactttaccg
acggtcatcagcccgggaagcctgactggtcagaggcaagccgattcgtt 2100
tgccagtagtcgggcccttcggactgaccagtctccgttcggctaagcaa
gccttcaccatgaaggacgaaaccaaaggcgagatctacgtggccttcaa 2150
cggaagtggtacttcctgctttggtttccgctctagatgcaccggaagtt
caccagtcaccttccggtggttgtcgggcttccagagcgctctgggttcc 2200
gtggtcagtggaaggccaccaacagcccgaaggtctcgcgagacccaagg
gatgggagccggtggtggacaccggcaaggaggcaccatatgacttcctc 2250
ctaccctcggccaccacctgtggccgttcctccgtggtatactgaaggag
accgatggcctaccagatcgtgctgtcaccgtctaccagttctctcattt 2300
tggctaccggatggtctagcacgacagtggcagatggtcaagagagtaaa
cctcaactccaatctctatcctatgctcagctactcctccatcatccttg 2350
ggagttgaggttagagataggatacgagtcgatgaggaggtagtaggaac
tattgcgccctgatgtctga 2370
ataacgcgggactacagact
No forbidden restriction sites.
Primers
Forward: gat gaattc gcggccgc t tctag atg gcg cag ctc ccc tg
Reverse: gct actagt a tta tta gac atc agg gcg caa tac
