Team:Paris/Construction

From 2008.igem.org

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|- style="background: #649cd7; text-align: center;"
|- style="background: #649cd7; text-align: center;"
|width=30%| Construction
|width=30%| Construction
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|width=50%| Description
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|width=55%| Description
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|width=10%| Validation Date
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|width=15%| Validation Date
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|width=10%| Link to Notebook
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA+)
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-
|align="center"|
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA - YFP Tripart (LVA-)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
|align="center"|  
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-
|align="center"|
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP
|align="center"|  
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-
|align="center"|
 
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP
|align="center"|  
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-
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|-
|-
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center>
|colspan=4 style="background: #649cd7" | <center>'''OSCILLATION'''</center>
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
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|-
|-
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center>
|colspan=4 style="background: #649cd7" | <center>'''SYNCHRONISATION'''</center>
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
|align="center"|  
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
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-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
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|-
|-
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center>
|colspan=4 style="background: #649cd7" | <center>'''NEW GENE AND PROMOTER BIOBRICKS'''</center>
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC
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|}
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Revision as of 02:11, 30 October 2008

FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+)
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA+)
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA-)
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+)
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA)
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP
OSCILLATION
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(+ RBS WT)-EnvZ*
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+ RBS WT)-EnvZ*-GFP generator
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(+ RBS WT)-EnvZ*
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator
Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ*
SYNCHRONISATION
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA)
Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter
Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP
NEW GENE AND PROMOTER BIOBRICKS
Part icon regulatory.png
pFliA
Part icon regulatory.png
pFliL (+RBS WT)
Part icon regulatory.png
pFlgA (+RBS WT)
Part icon regulatory.png
pFlgB (+RBS WT)
Part icon regulatory.png
pFlhB (+RBS WT)
Icon coding.png
EnvZ*
Icon coding.png
FliA
Icon coding.png
FlhDC
Retrieved from "http://2008.igem.org/Team:Paris/Construction"