Project
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- | <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Tianjin">Parts contributed to the registry</a><br></td> | + | <p align="left"><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Tianjin">Parts contributed to the registry</a><br> |
+ | </p></td> | ||
<td height="46" bgcolor="#03438A" class=" STYLE13"><img name="" src="https://static.igem.org/mediawiki/2008/4/45/Zong.gif" width="350" height="350" alt=""></td> | <td height="46" bgcolor="#03438A" class=" STYLE13"><img name="" src="https://static.igem.org/mediawiki/2008/4/45/Zong.gif" width="350" height="350" alt=""></td> | ||
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<p class="STYLE7">When induced, the expressed CCDB will lead to the Programed Cell Death (PCD). </p> | <p class="STYLE7">When induced, the expressed CCDB will lead to the Programed Cell Death (PCD). </p> | ||
<p class="STYLE7">In order to realize the linkage of GENE 1 with GENE 2, we will introduce the new plasmid containing the desired GENE2 to the survival cells, in which the plasmids containing GENE 1 will behave as the new Receptor plasmid. Very similarly recombination between the <em>attB2 </em>and<em> attP2 </em>and the cleavage between the two <em>loxp </em> sites will be performed, and plasmids containing the linked GENE1 and GENE2 will be selected when the promoter expresses CCDB is induced. </p> | <p class="STYLE7">In order to realize the linkage of GENE 1 with GENE 2, we will introduce the new plasmid containing the desired GENE2 to the survival cells, in which the plasmids containing GENE 1 will behave as the new Receptor plasmid. Very similarly recombination between the <em>attB2 </em>and<em> attP2 </em>and the cleavage between the two <em>loxp </em> sites will be performed, and plasmids containing the linked GENE1 and GENE2 will be selected when the promoter expresses CCDB is induced. </p> | ||
- | <p class="STYLE7">The reason for us to use two sets of<em> attB/attP</em> specific sites is to avoid the combination within one module.</p> | + | <p class="STYLE7">The reason for us to use two sets of<em> attB/attP</em> specific sites is to avoid the combination within one module.</p> </td> |
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</tr> | </tr> | ||
Revision as of 02:31, 30 October 2008
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This idea was inspired by the theory of Prisoner’s Dilemma. As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with each other by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival. |
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