Team:Princeton/ListofPlasmidDesigns

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Revision as of 03:05, 30 October 2008

PRINCETON IGEM 2008

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The highlight code is as follows:

style="background:yellow; color:black" | yellow: overlap of primers with the DNA
style="background:green; color:black" |dark green: restriction site
style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand.
style="background:cyan; color:black" |light blue: kozak sequence.
style="background:teal; color:black" |dark blue: stop codon.
style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell)
style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.

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