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Team:Princeton/ListofPlasmidDesigns
From 2008.igem.org
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[https://2008.igem.org/Media:pLV-TRE-Ngn1-EYFP-Ubc-Bla.pdf pLV-TRE-Ngn1-EYFP-Ubc-Bla] | [https://2008.igem.org/Media:pLV-TRE-Ngn1-EYFP-Ubc-Bla.pdf pLV-TRE-Ngn1-EYFP-Ubc-Bla] | ||
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| + | [https://2008.igem.org/Media:pLV-TRE-Ngn1-EYFP-2A-mKate-Ubc-Bla.pdf pLV-TRE-Ngn1-EYFP-2A-mKate-Ubc-Bla] | ||
[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro] | [https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-2A-LuxR-IRES-Puro] | ||
[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro] | [https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro] | ||
Revision as of 03:18, 30 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
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The highlight code is as follows:
style="background:yellow; color:black" | yellow: overlap of primers with the DNA style="background:green; color:black" |dark green: restriction site style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand. style="background:cyan; color:black" |light blue: kozak sequence. style="background:teal; color:black" |dark blue: stop codon. style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell) style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer.
