Team:BrownTwo/Limiter/altdesign

(Difference between revisions)

Revision as of 03:58, 30 October 2008

Alternative construction and design possiblities

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 Alternative construction and design possiblities
-*up-limiter
+*up-limiter - upregulates a gene when the expression falls below a certain level
-*dual limiter
+*dual limiter - regulates two genes simultaneously
 *limiter device as a regulatory bridge between two pathways
-*killswitch
-*Smolke’s gal2 knockout scheme for using the GAL1 promoter
-*Finding additional inducible/repressible promoters to use in the system
-*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
-In order to modify our limiter to regulate an endogenous gene network, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene.
-A handful of these parts were available in the Registry, but we found it necessary to
-Modularity –
-Stipulation:  If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.
-modifications to current scheme:
-Possible further modifications to these transcription factors include the use of different regulation domains,
-Involves the consideration of multiple binding sites