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| | <td colspan="2" class="subHeader">Designed by:</td> | | <td colspan="2" class="subHeader">Designed by:</td> |
| - | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> | + | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> |
| | </tr> | | </tr> |
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| | <tr> | | <tr> |
| | <td colspan="2" class="subHeader">Designed by:</td> | | <td colspan="2" class="subHeader">Designed by:</td> |
| - | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> | + | <td colspan="2" rowspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</div></td> |
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| | <td colspan="2" class="subHeader">Designed by:</td> | | <td colspan="2" class="subHeader">Designed by:</td> |
| - | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> | + | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> |
| | </tr> | | </tr> |
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| | <tr> | | <tr> |
| | <td colspan="2" class="subHeader">Designed by:</td> | | <td colspan="2" class="subHeader">Designed by:</td> |
| - | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> | + | <td colspan="2" class="bodyText"><div align="justify">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo</div></td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
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LCG-UNAM-Mexico |
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| iGEM 2008 TEAM |
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Parts Submitted to the Registry |
Constructed Parts
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| The following are the bioparts that we designed for our project: |
| Constitutive weak promoter of lacZ |
| LacZ constitutive promoter, without operators. |
| 38 bp |
| Miguel Angel Ramirez-Romero |
| |
| Mutated LacZ promoter |
Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker |
| 38 bp |
| Miguel Angel Ramirez-Romero |
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| RcnR operator (represses RcnA) |
| Operator for RcnR(YohL) the repressor of nickel extrusion pump(RcnA) in E.coli |
| 83 bp |
| Libertad Pantoja |
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| RcnA (YohM) |
| Nickel efflux pump from E.coli |
| 833 bp |
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo |
| |
| Upper primer for aiiA with normal lacZ promoter |
Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter |
| 67 bp |
| Martin del Castillo V. |
| |
| Upper primer for aiiA with mutated lacZ promoter |
Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter |
| 67 bp |
| Martin del Castillo V. |
| |
| Upper primer for RcnA |
Upper primer used to add cI promoter upstream of the RcnR operator in the device cI promoter RcnR op RcnA |
| 73 bp |
| Libertad Pantoja |
| |
| Lower primer for RcnA (BBa_K119003) |
Lower primer for RcnA (BBa_K119003). Includes only the end of the RcnA coding region. |
| 21 bp |
| Libertad Pantoja |
| |
| cI promoter RcnR op RcnA |
This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion. |
| 969 bp |
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo |
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| LuxR_cI_aiiA_Normal lacZ promoter |
This device encodes CI, the protein which controls rcnA expression. It also contains luxR, which induces the expression of cI and aiiA. |
| 2873 bp |
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo |
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| LuxR_cI_aiiA_Mutated lacZ promoter |
This device encodes CI, the protein which controls rcnA. It also contains luxR, which induces the expression of cI and aiiA |
| 2873 bp |
Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,B. Mariana Reyes, Luis A. Arrola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo |
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