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Minnesota/26 June 2008
From 2008.igem.org
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Emartin9808 (Talk | contribs) (New page: {| |- |1. '''Glycerol Stock:''' |- |a. Add 900uL of glycerol to empty tube |- |b. Add 900uL of cells to the glycerol tubes |- |c. Immediately place tubes in a beaker that has ethanol and ...) |
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|c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | |c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | ||
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| - | |d. Place tubes in freezer @ -79C | + | |d. Place tubes in freezer @ -79C for future use. |
|} | |} | ||
Revision as of 15:34, 1 July 2008
| 1. Glycerol Stock: |
| a. Add 900uL of glycerol to empty tube |
| b. Add 900uL of cells to the glycerol tubes |
| c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. |
| d. Place tubes in freezer @ -79C for future use. |
