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Minnesota/26 June 2008
From 2008.igem.org
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|1. '''Glycerol Stock:''' | |1. '''Glycerol Stock:''' | ||
|- | |- | ||
| - | |a. Add 900uL of glycerol to empty tube | + | |a. Add 900uL of glycerol to empty tube. |
|- | |- | ||
| - | |b. Add 900uL of cells to the glycerol tubes | + | |b. Add 900uL of cells to the glycerol tubes. |
|- | |- | ||
|c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | |c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. | ||
Revision as of 15:34, 1 July 2008
| 1. Glycerol Stock: |
| a. Add 900uL of glycerol to empty tube. |
| b. Add 900uL of cells to the glycerol tubes. |
| c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes. |
| d. Place tubes in freezer @ -79C for future use. |
