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Team:University of Ottawa/26 June 2008
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Djedrysiak (Talk | contribs) (New page: __TOC__ '''Dan''' :'''PCR gel''' ::<li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and pu...) |
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::<li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify. | ::<li>The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify. | ||
::<li> After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products. | ::<li> After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products. | ||
| - | ::<li> S&D&T amplification products showed low DNA concentration, however it is still | + | ::<li> S&D&T amplification products showed low DNA concentration, however it is still within the usable range |
| + | :'''Digestion''' | ||
| + | ::<li> 0 and 1 A and B PCR products were digested with ClaI so that they could be ligated in various combinations | ||
Revision as of 17:20, 1 July 2008
Dan
- PCR gel
- The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify.
- After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products.
- S&D&T amplification products showed low DNA concentration, however it is still within the usable range
- Digestion
- 0 and 1 A and B PCR products were digested with ClaI so that they could be ligated in various combinations
