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Team:NTU-Singapore/Notebook/2 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: =Wednesday 2 July= *Arrival of E7+Imm Oligo **PCR E7+Imm *Attempted Ligation of Fe-GFP & LacI-Rbs34 **Failed Attempt due to faulty gel ***Loading dye was unable to keep DNA sample settled ...) |
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***Samples settled into the gel and started floating out of it. | ***Samples settled into the gel and started floating out of it. | ||
*Preliminary Speculation of problem is due to loading dye. | *Preliminary Speculation of problem is due to loading dye. | ||
| + | *Luchao, Hung: | ||
| + | **miniprep for pFe,pLacI,GFP and RBS 34 | ||
| + | **Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates). | ||
Revision as of 11:32, 2 July 2008
Wednesday 2 July
- Arrival of E7+Imm Oligo
- PCR E7+Imm
- Attempted Ligation of Fe-GFP & LacI-Rbs34
- Failed Attempt due to faulty gel
- Loading dye was unable to keep DNA sample settled at the bottom of the well
- Samples settled into the gel and started floating out of it.
- Failed Attempt due to faulty gel
- Preliminary Speculation of problem is due to loading dye.
- Luchao, Hung:
- miniprep for pFe,pLacI,GFP and RBS 34
- Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates).
