Team:KULeuven/Tools

From 2008.igem.org

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(→Digestion sheet)
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'''Usage:'''
'''Usage:'''
 +
<nowiki>
 +
{{:Team:KULeuven/Tools/Digestion
 +
|nr=D12
 +
|product=Cre ORF
 +
|matrix=pJ61047
 +
|enz1=Pst1
 +
|enz2=Xba1
 +
|size=1063bp
 +
|desc=Cre ORF extracted for further usage
 +
}}
 +
</nowiki>
 +
 +
'''Result:'''
 +
{{:Team:KULeuven/Tools/Digestion
 +
|nr=D12
 +
|product=Cre ORF
 +
|matrix=pJ61047
 +
|enz1=XbaI
 +
|enz2=PstI
 +
|size=1063bp
 +
|desc=Cre ORF extracted for further usage
 +
}}
==== [[Team:KULeuven/Tools/Ligation | Ligation sheet]] ====
==== [[Team:KULeuven/Tools/Ligation | Ligation sheet]] ====

Revision as of 13:45, 3 July 2008

Under Construction...

Contents

Templates

General

Navigation Bar

Usage: 

{{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage: 

{{:Team:KULeuven/Tools/PCR
 |tanneal=55
 |telong=2'30"
 |ncycles=30
 |buffer=5x 10μL
 |mgcl=10μM 0μL
 |dntp=10μM 1μL
 |fwprimername=6 LacZ-Fw
 |fwprimerconc=10μM 2.5μL
 |rvprimername=7 LacZ-Rv
 |rvprimerconc=10μM 2.5μL
 |water=34μL
 |polymerase=Taq 0.5μL
 |dna=0.5μL of E. coli DNA extract
 |size=952bp
 |success=YES/NO
 |image=pcrproducts_GE.jpg
 |lane=3+4
 }}

Result:

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet

Usage: 

 {{:Team:KULeuven/Tools/Digestion
 |nr=D12
 |product=Cre ORF
 |matrix=pJ61047
 |enz1=Pst1
 |enz2=Xba1
 |size=1063bp
 |desc=Cre ORF extracted for further usage
 }}

Result:

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D12 Cre ORF pJ61047 XbaI PstI 1063bp Cre ORF extracted for further usage

Ligation sheet

Usage:

CSS

Calendar CSS file

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