Team:The University of Alberta/3 July 2008
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(New page: ===Today=== '''Chris'''<br> *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the c...)
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(New page: ===Today=== '''Chris'''<br> *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the c...)
Newer edit →
Revision as of 16:58, 3 July 2008
Today
Chris
- After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure.
Jason
- Gel purified PCR products from the colony PCR yesterday
- Sequenced the purified products
Saima
- Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls)
Tom
- Made two SDS-PAGE gels for Chris to use (see above)