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Team:NTU-Singapore/Notebook/4 July 2008
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(New page: =Friday 4 July= *Hung, Darius: **Ligation of E7 into empty plasmid vector extracted from GFP plasmid. ***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recog...)
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(New page: =Friday 4 July= *Hung, Darius: **Ligation of E7 into empty plasmid vector extracted from GFP plasmid. ***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recog...)
Newer edit →
Revision as of 18:10, 5 July 2008
Friday 4 July
- Hung, Darius:
- Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
- Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
- Gel extraction of E7 bands (around 2kb) to remove all the oligonucleotides.
- Purification for E7 after extracted.
- Digestion of GFP by EcoRI and PstI. Incubation for 3 hours.
- Digestion of purified E7 by EcoRI and PstI. Incubation for 3 hours.
- Gel electrophoresis and gel extraction for GFP to get the empty plasmid vector (around 2kb).
- PCR purification for digested E7 to remove all the enzymes, buffers and small nucleotides.
- Ligation of empty plasmid (from GFP) and E7 insert.
- Transformation and cloning into homemade top10 cells.
- Lu Chao, Hung: transformation and cell cloning of LacI-GFP and LacI-RFP again.
- Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
- Lu Chao: inoculation of LacI-RBS cells.
- Choon Kit: documentation of lab protocols.
