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Team:Hawaii/Notebook/2008-07- 7
From 2008.igem.org
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:* Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3 | :* Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3 | ||
| - | ===[Team:Hawaii/Initial_Synth._Oligo_Assembly|Oligonucleotide experiment]=== | + | ===[[Team:Hawaii/Initial_Synth._Oligo_Assembly|Oligonucleotide experiment]]=== |
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
Revision as of 04:56, 8 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Biobrick Extraction
- Margaret
- Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3
Oligonucleotide experiment
- Grace
- Ligated annealed products using 10-2 dilution of products
- Reran on EtBr stained 4% agarose gel (result: light bands for annealed products, correct size; no bands for ligated product)
- Ran annealed products (10-1 dilution) on SYBR Safe stained 2% agarose gel
- Restriction digested ligation from 7/3/08 using XbaI and PstI in NEBuffer 3
- Reran on EtBR stained 4% agarose gel
Plasmid prep
- Krystle
- Grew up E. coli from cryostock and performed an alkaline lysis plasmid prep for C0012
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
