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Team:Hawaii/Notebook/2008-07- 7
From 2008.igem.org
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:* Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3 | :* Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3 | ||
| + | :* Transformed into E. coli | ||
| + | :* 7/08/08: checked and there are no transformants | ||
===[[Team:Hawaii/Initial_Synth._Oligo_Assembly|Oligonucleotide experiment]]=== | ===[[Team:Hawaii/Initial_Synth._Oligo_Assembly|Oligonucleotide experiment]]=== | ||
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:* Grew up ''E. coli'' from cryostock and performed an alkaline lysis plasmid prep for C0012 | :* Grew up ''E. coli'' from cryostock and performed an alkaline lysis plasmid prep for C0012 | ||
| + | ===PCR of pRL1383a parts=== | ||
| + | :<strong> Margaret</strong> | ||
| + | |||
| + | :* Amplification of ''rep'' and ''mob'' regions. Neither of these parts came out very well, so trying them with red and green taq. | ||
= Discussion = | = Discussion = | ||
Revision as of 20:03, 8 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Biobrick Extraction
- Margaret
- Extracted Biobricks BBa_E0240, BBa_I4032, BBa_pSB3K3
- Transformed into E. coli
- 7/08/08: checked and there are no transformants
Oligonucleotide experiment
- Grace
- Ligated annealed products using 10-2 dilution of products
- Reran on EtBr stained 4% agarose gel (result: light bands for annealed products, correct size; no bands for ligated product)
- Ran annealed products (10-1 dilution) on SYBR Safe stained 2% agarose gel
- Restriction digested ligation from 7/3/08 using XbaI and PstI in NEBuffer 3
- Reran on EtBR stained 4% agarose gel
Plasmid prep
- Krystle
- Grew up E. coli from cryostock and performed an alkaline lysis plasmid prep for C0012
PCR of pRL1383a parts
- Margaret
- Amplification of rep and mob regions. Neither of these parts came out very well, so trying them with red and green taq.
Discussion
Quote of the Day
"I assume if there are jellyfish, it's not completely pelleted?" - KS
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
