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Minnesota/10 July 2008
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|1. '''Dephosphorylation:''' Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. | |1. '''Dephosphorylation:''' Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. | ||
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| + | {|border="1" align="left" | ||
| + | |- | ||
| + | !| Gene !!10x Buffer !!H20 !!DNA !!Phosphotase | ||
| + | |- | ||
| + | |Base Vector ||5.6uL || -- ||50.0uL ||1.0uL | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | |- | ||
| + | |NOTE: Total volume in dephosphorylation = 56.6uL | ||
Revision as of 16:04, 10 July 2008
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1. Dephosphorylation: Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product.
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| NOTE: Total volume in dephosphorylation = 56.6uL |
