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Purdue/10 July 2008
From 2008.igem.org
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After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. | After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. | ||
| - | Bead plated, and let incubate at 37C o/n. | + | Bead plated control (pUC19), LacZalpha, and LacY genes, and let incubate at 37C o/n. |
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| + | Found via email from Chris French (Edinburgh) that RFP will make a visible red color. Therefore, we don't need any fancy MacConkey agar combined with a lac operon. Yay! | ||
| + | |||
| + | Revision 10385: First reporter = phr + RFP, 2nd reporter = SOS (recA) + LacZ. We'll still put X-gal in the substrate. | ||
'''Edited by Janie Stine''' | '''Edited by Janie Stine''' | ||
Revision as of 16:37, 10 July 2008
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After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. Bead plated control (pUC19), LacZalpha, and LacY genes, and let incubate at 37C o/n.
Found via email from Chris French (Edinburgh) that RFP will make a visible red color. Therefore, we don't need any fancy MacConkey agar combined with a lac operon. Yay!
Revision 10385: First reporter = phr + RFP, 2nd reporter = SOS (recA) + LacZ. We'll still put X-gal in the substrate.
Edited by Janie Stine
