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Team:Hawaii/Notebook/2008-07-14
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===PCR=== :<strong> Grace</strong> :* 25 μl PCR reactions for GFP site directed mutagenesis and extraction of Biobrick...)
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===PCR=== :<strong> Grace</strong> :* 25 μl PCR reactions for GFP site directed mutagenesis and extraction of Biobrick...)
Newer edit →
Revision as of 02:15, 15 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR
- Grace
- 25 μl PCR reactions for GFP site directed mutagenesis and extraction of Biobrick pertinent sites from BBa_B0034
Made stocks
- Grace
- Made two 500mL batches of SOB
- Made 20 LB + amp100 plates
Transformed DH5α with ligations of a Biobrick vector with pnir, slr2016-1, slr2016-2, pilA, or C0012
- Grace
- Transformed using 5 μl of 1:1 and 1:10 ligation dilutions
- Transformed using 1 μl vector only (negative control)
- Incubated on ice 10 min as opposed to the recommended 30 min. by iGEM
Transformed DH5α with mutated GFP and new Biobrick vector constructs
- Grace
- Constructs:
- PCR'd GFP fusion brick ligated with Biobrick vector derived from BBa_C0012
- Biobrick pertinent segment ligated with pRL1383a
- Constructs were restriction digested, agarose gel purified, and ligated together using Quick Ligase
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
