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Team:Hawaii/Notebook/2008-07-14
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===PCR=== :<strong> Grace</strong> :* 25 μl PCR reactions for GFP site directed mutagenesis and extraction of Biobrick...) |
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::* Constructs were restriction digested, agarose gel purified, and ligated together using Quick Ligase | ::* Constructs were restriction digested, agarose gel purified, and ligated together using Quick Ligase | ||
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| + | ===Transformed DB3.1=== | ||
| + | :<strong> Margaret</strong> | ||
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| + | :*Transformed: pSB3K3, pSB1A2, pSB1AK3, (+)pUC18, (-)nothing | ||
= Discussion = | = Discussion = | ||
Revision as of 03:32, 15 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR
- Grace
- 25 μl PCR reactions for GFP site directed mutagenesis and extraction of Biobrick pertinent sites from BBa_B0034
Made stocks
- Grace
- Made two 500mL batches of SOB
- Made 20 LB + amp100 plates
Transformed DH5α with ligations of a Biobrick vector with pnir, slr2016-1, slr2016-2, pilA, or C0012
- Grace
- Transformed using 5 μl of 1:1 and 1:10 ligation dilutions
- Transformed using 1 μl vector only (negative control)
- Incubated on ice 10 min as opposed to the recommended 30 min. by iGEM
Transformed DH5α with mutated GFP and new Biobrick vector constructs
- Grace
- Constructs:
- PCR'd GFP fusion brick ligated with Biobrick vector derived from BBa_C0012
- Biobrick pertinent segment ligated with pRL1383a
- Constructs were restriction digested, agarose gel purified, and ligated together using Quick Ligase
Transformed DB3.1
- Margaret
- Transformed: pSB3K3, pSB1A2, pSB1AK3, (+)pUC18, (-)nothing
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
