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EPF-Lausanne/11 July 2008
From 2008.igem.org
(Difference between revisions)
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SOC, and the other one is not diluted. | SOC, and the other one is not diluted. | ||
- Let them grow at 37 degrees overnight and then put at 4 degrees in the fridge. | - Let them grow at 37 degrees overnight and then put at 4 degrees in the fridge. | ||
| + | |||
| + | Results : The control, predator1 and predator 2 grow very well. The prey 1 and prey 2 hasn't grow. | ||
| + | We will try again with the prey 1 and prey 2. | ||
- We prepare some LB plates with different antibiotics (Amp, Cm, Kan) | - We prepare some LB plates with different antibiotics (Amp, Cm, Kan) | ||
Revision as of 10:14, 15 July 2008
- We have found Top10 cells! We grow them at 37 degrees on LB plates overnight, and then put at 4 degrees in the fridge.
- We do some transformations,following the protocol of Invitrogen. :
- Cells : DH5Alpha - Plasmids from the predator-prey systems. Predator 1, Predator 2, Prey 1, Prey 2, and pUC19 for the control. We do always two plates. One is diluted 1/10 in SOC, and the other one is not diluted. - Let them grow at 37 degrees overnight and then put at 4 degrees in the fridge.
Results : The control, predator1 and predator 2 grow very well. The prey 1 and prey 2 hasn't grow. We will try again with the prey 1 and prey 2.
- We prepare some LB plates with different antibiotics (Amp, Cm, Kan)
