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Team:Hawaii/Notebook/2008-07-15
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Oligonucleotide experiment, continued=== :<strong> Grace</strong> :* Ran...) |
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:* Counted colonies from yesterday's pnir, slr2016, pilA assembly transformation (see [[Team:Hawaii/Initial_Synth._Oligo_Assembly#Sixth_attempt|experiment]]) | :* Counted colonies from yesterday's pnir, slr2016, pilA assembly transformation (see [[Team:Hawaii/Initial_Synth._Oligo_Assembly#Sixth_attempt|experiment]]) | ||
:* Colony PCR'd colonies to verify presence of desired products | :* Colony PCR'd colonies to verify presence of desired products | ||
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| + | ===Transformation=== | ||
| + | <strong>Margaret</strong> | ||
| + | |||
| + | :*Transformation of DB3.1 competent cells pSB3K3, pSB1A2, pSB1AK3, (+)pUC18, (-) no plasmid, because transformation from 7/14/08 yielded only 1 colony from pSB3K3, nothing from pSB1A2 & pSB1AK3. | ||
= Discussion = | = Discussion = | ||
Revision as of 01:12, 16 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Oligonucleotide experiment, continued
- Grace
- Ran overnight RE digests of pRL1383a and BBa_C0012 on EtBr stained 2% agarose gel at 95V for 1 hour
- BBa_C0012 vector okay
- pRL1383a vector band ~9kb; no band observed at ~350bp. RE did not cut?
- Gel purified pRl1383a, BBa_C0012, GFP fusion, and Biobrick segments
- Used nanodrop spectrometer to determine DNA concentration (below)
- Ligated 7 μl Biobrick segment with 0.5 μl pRL1383a and 7 μl GFP fusion with 2 μl BBa_C0012 vector in 20 μl ligation reactions
- Transformed DH5α using 10 μl ligation reactions
- Counted colonies from yesterday's pnir, slr2016, pilA assembly transformation (see experiment)
- Colony PCR'd colonies to verify presence of desired products
Transformation
Margaret
- Transformation of DB3.1 competent cells pSB3K3, pSB1A2, pSB1AK3, (+)pUC18, (-) no plasmid, because transformation from 7/14/08 yielded only 1 colony from pSB3K3, nothing from pSB1A2 & pSB1AK3.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
