Team:KULeuven/Parts

From 2008.igem.org

(Difference between revisions)
(→Submitted Parts)
Line 5: Line 5:
== Submitted Parts ==
== Submitted Parts ==
-
* '''K145000''': '''--> K145100''' eCFP with LVA tag under OmpR control  
+
{|  style="width:95%"
-
* '''K145001''': T7 RNA polymerase with LVA tag ''(part) (in filter plasmid 2)''
+
|-
-
* '''K145002''': T7 RNA Polymerase with LVA tag under OmpF control ''(composite part) (filter plasmid 1)''
+
! Part number
-
* '''K145003''': '''--> K145102''' RiboKey 3d under control of T7 RNA polymerase promotor  
+
! Replaced by
-
* '''K145004''': '''--> K145104'''T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.  
+
! Description
-
* '''K145005''': cI under control of a dual promotor and a RiboLock. ''???''
+
! Type
-
* '''K145006''': '''--> K145106''' Lactonase under PRM control  
+
! Component
-
* '''K145007''': '''--> K145107''' Pulse Generator  
+
|-
-
* '''K145008''': '''--> K145108''' Constitutive production of LuxR  
+
| '''K145000'''
-
* '''K145009''': '''--> K145109''' ccdB cell death gene under control of an activating Lux PR
+
| '''K145100'''
-
* '''K145010''': '''--> K145110''' Killing switch
+
| eCFP with LVA tag under OmpR control
-
* '''K145011''': Light sensor to provide dummy input for the system ''(= input)''
+
| part
-
* '''K145013''': antisense LuxI  
+
| input
-
* '''K145100''': eCFP with LVA tag under control of a OmpF promotor
+
|-
-
* '''K145101''': Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor.  
+
| '''K145001'''
-
* '''K145102''': RiboKey3d under control of OmpF promotor ''(in filter)''
+
| /
-
* '''K145103''': T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d  
+
| T7 RNA polymerase with LVA tag
-
* '''K145104''': AND part, T7 and RiboKey3d together will produce LacI. This will repress transcription of LuxI from a pLac promotor ''(= inverter)''
+
| part
-
* '''K145105''': cI under T7 and PR dual promotor system. Should generate a pulse of cI.  
+
| filter plasmid 2
-
* '''K145106''': Lactonase under PRM control  
+
|-
-
* '''K145107''': '''--> K145117''' Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present  
+
| '''K145002'''
-
* '''K145108''': LuxR and TetR under control of a constitutive promotor.  
+
| /
-
* '''K145109''': ccdB cell death gene under the control of a LuxPR promotor.  
+
| T7 RNA Polymerase with LVA tag under OmpF control
-
* '''K145110''': Complete cell death mechanism. Combination of BBa_ K145108 and BBa_K145109.  
+
| composite part
-
* '''K145111''': Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor  
+
| filter plasmid 1
-
* '''K145112''': cI under T7 and PR dual promotor system. Should generate a pulse of cI.  
+
|-
-
* '''K145115''': cI under T7 and PR dual promotor system. Should generate a pulse of cI.  
+
| '''K145003'''
-
* '''K145117''': Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
+
| '''K145102'''
 +
| RiboKey 3d under control of T7 RNA polymerase promotor
 +
| /
 +
| /
 +
|-
 +
| '''K145004'''
 +
| '''K145104'''
 +
|  T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.
 +
| /
 +
| /
 +
|-
 +
| '''K145005'''
 +
|
 +
| cI under control of a dual promotor and a RiboLock.
 +
|
 +
|
 +
|-
 +
| '''K145006'''
 +
| '''K145106'''
 +
| Lactonase under PRM control
 +
|
 +
|
 +
|-
 +
| '''K145007'''
 +
| '''K145107'''
 +
| Pulse Generator
 +
|
 +
|
 +
|-
 +
| '''K145008'''
 +
| '''K145108'''
 +
| Constitutive production of LuxR
 +
|
 +
|
 +
|-
 +
| '''K145009'''
 +
| '''K145109'''
 +
| ccdB cell death gene under control of an activating Lux PR
 +
|
 +
|
 +
|-
 +
| '''K145010'''
 +
| '''K145110'''
 +
| Killing switch
 +
|
 +
|
 +
|-
 +
| '''K145011'''
 +
|
 +
| Light sensor to provide dummy input for the system
 +
|
 +
| input
 +
|-
 +
| '''K145013'''
 +
|
 +
| antisense LuxI
 +
|
 +
|
 +
|-
 +
| '''K145100'''
 +
|
 +
| eCFP with LVA tag under control of a OmpF promotor
 +
|
 +
|
 +
|-
 +
| '''K145101'''
 +
|
 +
| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor.  
 +
|
 +
|
 +
|-
 +
| '''K145102'''
 +
|
 +
| RiboKey3d under control of OmpF promotor
 +
|
 +
|filter
 +
|-
 +
| '''K145103'''
 +
|
 +
| T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d
 +
|
 +
|
 +
|-
 +
| '''K145104'''
 +
|
 +
| AND part, T7 and RiboKey3d together will produce LacI. This will repress transcription of LuxI from a pLac promotor
 +
|
 +
| inverter
 +
|-
 +
| '''K145105'''
 +
|
 +
| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 +
|
 +
|
 +
|-
 +
| '''K145106'''
 +
|
 +
| Lactonase under PRM control
 +
|
 +
|
 +
|-
 +
| '''K145107'''
 +
| '''K145117'''
 +
| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
 +
|
 +
|
 +
|-
 +
| '''K145108'''
 +
|
 +
| LuxR and TetR under control of a constitutive promotor.
 +
|
 +
|
 +
|-
 +
| '''K145109'''
 +
|
 +
| ccdB cell death gene under the control of a LuxPR promotor.
 +
|
 +
|
 +
|-
 +
| '''K145110'''
 +
|
 +
| Complete cell death mechanism. Combination of '''BBa_ K145108''' and '''BBa_K145109'''.
 +
|
 +
|
 +
|-
 +
| '''K145111'''
 +
|
 +
| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor  
 +
|
 +
|-
 +
| '''K145112'''
 +
|
 +
| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 +
|
 +
|
 +
|-
 +
| '''K145115'''
 +
|
 +
| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 +
|
 +
|
 +
|-
 +
| '''K145117'''
 +
|
 +
| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
 +
|
 +
|
 +
|}
 +
 
 +
 
==The Big Sheme of Used Parts==
==The Big Sheme of Used Parts==
[[Image:THE_BIG_SCHEME.xls]]
[[Image:THE_BIG_SCHEME.xls]]

Revision as of 09:18, 17 July 2008

Result:

To have a dropdown for your own team copy over all of the content below. It might be needed to adjust a thing or two. Comments have been added throughout the code what must be changed.

Inspirational websites:

  • [http://javascript-array.com/scripts/simple_drop_down_menu/ Most basic dropdown menu, with no submenu's]
  • [http://jquery.com/ The javascript library used to add effects and optimise the dropdown]
  • [http://www.tyssendesign.com.au/articles/css/centering-a-dropdown-menu/ How to center a ul list effectively]

The dropdown has been created and developed by the KULeuven team. 

<html>

<style type="text/css">
#content {z-index:4;}
#ddwrapper * {z-index:8 !important;}

div#ddwrapper {
	margin:0;
	padding:0;
	height:28px;
	width:945px;				/* change to adjust imperfections in width */
}
div#ddnav {
	margin:0 auto;				/* needed to center the dropdown */
	padding:0;
	top:5px;
	/* width: 965px */
	visibility:hidden;			/* dropdown is hidden until properly initalised by javascript */
}
div#ddtoggle {
	margin:0;
	position:fixed;
	right:2px;
	top:15px;
	height:10px;
	width:10px;
	z-index:100;
}

#ddnav ul {
	display:table-row;			/* works only for firefox, later adjusted by javascript for IE */
	margin:0 auto;
	padding:0;
}
#ddnav ul li {
	display:table-cell;			/* works only for firefox, later adjusted by javascript for IE */
	list-style:none;
	margin:0;
	padding:0 !important;
	border-right:1px solid #FFF;		/* creates illusion of spacing between tabs */
}
#ddnav ul li:last-child{border-right:none;}
#ddnav a{
	display:block;
	margin:0;
	padding:4px 14px;			/* play with dimensions for size of tabs */
	background-color:#075A90;		/* background color for tabs */
	color:#FFF !important;			/* font color for text in tabs */
	text-align:center;			/* aligning for text in tabs */
	text-decoration:none !important;
	font:bold 10pt Trebuchet MS;		/* font properties for text in tabs */
	outline:0;
}
#ddnav ul li a:hover {background-color:#99CCFF;}/* background color for tabs on mouseover */
#ddnav li a:active {outline:none;}		/* remove standard dotted border for links when clicked (IE) */
#ddnav li a:focus {-moz-outline-style:none;}	/* remove standard dotted border for links when clicked (FF) */
#ddnav div {
	display:none;
	position:absolute;
	width:9em;
	background-color:#000;			/* bug solution, do not change ! */
	border:1px solid #5970B2;		/* border color for dropdown menus */
	opacity:0.9;				/* transparancy of the dropdown menus (FF) */
	filter:alpha(opacity=90);		/* transparancy of the dropdown menus (IE) */
}
#ddnav div a {
	display:block;
	padding:5px 10px;			/* play with dimensions of block element in dropdown menus */
	position:relative;
	font:normal 8pt arial;			/* font properties for text in dropdown menus */
	text-align:left;			/* aligning of text in dropdown menus */
	cursor:pointer;
}
#ddnav div a:hover, #ddnav span a:hover {color:#000 !important;}	/* text color on mouseover */
#ddnav span div {
	position:relative;
	border:none;
	border-bottom:2px groove #5970B2;	/* separator for submenus, groove does not work in FF */
	opacity:1.0;				/* avoid stacking transparancy for submenus (FF) */
	filter:alpha(opacity=100);		/* avoid stacking transparancy for submenus (IE) */
}

/* may want to upload the following pictures to a new location */
.expand {background: url('https://static.igem.org/mediawiki/2008/e/ef/Icon-expand.png') no-repeat 95% 50%;}
.collapse {background: url('https://static.igem.org/mediawiki/2008/c/cd/Icon-collapse.png') no-repeat 95% 50%;}

.docked {background: #99ccff url("https://static.igem.org/mediawiki/2008/6/62/Ddnavundock.png") no-repeat 50% 50%;}
.undocked {background: #99ccff url("https://static.igem.org/mediawiki/2008/e/e4/Ddnavdock.png") no-repeat 50% 50%;}
</style>

<!-- IMPORTANT: save following script under a personalized webspace or download the library at www.jquery.com -->
<script type="text/javascript" src="http://student.kuleuven.be/~s0173901/wiki/js/jquery.js"></script>
<script type="text/javascript">
	function ddnav() {
		$('#ddnav ul li').hover(
			function () {
				$(this).find('div:first').css('display','block');},
			function () {
				$(this).find('div:first').css('display','none');}
		);
	}
			
	function ddnavsub() {
		$('#ddnav span > a').toggle(
			function () {
				$(this).removeClass("#ddnav expand").addClass("#ddnav collapse");
				$(this).parent().find('div:first').slideDown('fast');
				$(this).hover(
					function (){$(this).css('background-color','#99AAFF');},
					function (){$(this).css('background-color','#99AAFF');});},
			function () {
				$(this).removeClass("#ddnav collapse").addClass("#ddnav expand");
				$(this).parent().find('div:first').css('display','none');
				$(this).hover(
					function (){$(this).css('background-color','#99CCFF');},
					function (){$(this).css('background-color','#075A90');});}
		).addClass("#ddnav expand");
	}
	
	/* If you wish to omit the docking feature, remove following function ddtoggle() */	
	function ddtoggle() {
		$('#ddtoggle').toggle(
			function () {
				$(this).removeClass('undocked').addClass('docked');
				$('#ddnav').css('position','fixed');},
			function () {
				$(this).removeClass('docked').addClass('undocked');
				$('#ddnav').css('position','static');}
		);
	}

	function ddalign() {
		var _windowWidth = $(window).width();
		var _leftOffset = (_windowWidth - 965)/2;

		$('div#ddnav').css('left',_leftOffset);
	}
			
	function ddmsie() {
		$('#ddnav a').hover(
			function () {$(this).css('background-color','#99ccff');},
			function () {$(this).css('background-color','#075a90');}
		);
				
		/* toggle doesn't work yet */
		$('#ddtoggle').css('display','none');
				
		$('#ddnav ul').css('display','inline-block');
		$('#ddnav ul li').css('display','inline');
		$('#ddnav ul li').css('position','relative');
		$('#ddnav ul li>a').css('display','inline-block');
		$('#ddnav ul li>a').css('margin-right','-4px');
				
		$('#ddnav div').css('left','0');
		$('#ddnav div').css('top','100%');
		$('#ddnav span div').css('top','0');
	}
			
	function ddmozilla() {
		ddtoggle();
		$(window).bind('resize', function() {ddalign();});
	}

	$(function () {
		ddnav();
		ddnavsub();

		if(jQuery.browser.msie) ddmsie();
		if(jQuery.browser.mozilla) ddmozilla();

		$('#ddnav').css('visibility','visible');
	});
</script>

<!-- If you wish to omit the docking feature omit following line (div with id ddtoggle) -->
<div id="ddtoggle" class="undocked"></div>
<div id="ddwrapper">
<!-- Here the actual links are defined, simply replace with your own links in the appropriate sections -->
<div id="ddnav" align="center">
<ul>
	<li>
		<a href="https://2008.igem.org/Team:KULeuven">Home</a>
	</li>
	<li>
		<a>The Team</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Team/LabsandGroups">Research Labs and Groups</a>
			<a href="https://2008.igem.org/Team:KULeuven/Team/Students">Students</a>
			<a href="https://2008.igem.org/Team:KULeuven/Team/Instructors">Instructors</a>
			<a href="https://2008.igem.org/Team:KULeuven/Team/Advisors">Advisors</a>
                        <a href="https://2008.igem.org/Team:KULeuven/Team/Pictures">Pictures</a>
		</div>
	</li>
	<li>
		<a>The Project</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Project">Summary</a>
			<span>
				<a>Components</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Input">Input</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Output">Output</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Filter">Filter</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Inverter">Invertimer</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Reset">Reset</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/CellDeath">Cell Death</a>
					<a href="https://2008.igem.org/Team:KULeuven/Project/Memory">Memory</a>
				</div>
			</span>
                        <a href="https://2008.igem.org/Team:KULeuven/Evaluation">End Evaluation</a>
			<a href="https://2008.igem.org/Team:KULeuven/Literature">Literature</a>
                        <a href="https://2008.igem.org/Team:KULeuven/Brainstorm">Brainstorm</a>
		</div>
	</li>
        <li>
		<a>Ethics</a>
		<div>

		</div>
	</li>
	<li>
		<a>Submitted Parts</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Parts">Listing</a>
			<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=KULeuven">Sandbox</a>
		</div>
	</li>
	<li>
		<a>Modeling</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Model/Overview">Overview</a>
			<a href="https://2008.igem.org/Team:KULeuven/Model/KineticConstants">Kinetic Constants</a>
			<span>
				<a>Components</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Model/Output">Output</a>
					<a href="https://2008.igem.org/Team:KULeuven/Model/Filter">Filter</a>
					<a href="https://2008.igem.org/Team:KULeuven/Model/Inverter">Invertimer</a>
					<a href="https://2008.igem.org/Team:KULeuven/Model/Reset">Reset</a>
					<a href="https://2008.igem.org/Team:KULeuven/Model/CellDeath">Cell Death</a>
					<a href="https://2008.igem.org/Team:KULeuven/Model/Memory">Memory</a>
				</div>
			</span>
			<a href="https://2008.igem.org/Team:KULeuven/Model/FullModel">Full Model</a>
                        <a href="https://2008.igem.org/Team:KULeuven/Model/Sensitivity">Sensitivity Analysis</a>
			<a href="https://2008.igem.org/Team:KULeuven/Model/MultiCell">Multi-cell Model</a>
			<a href="https://2008.igem.org/Team:KULeuven/Model/Diffusion">Diffusion</a>
		</div>
	</li>
	<li>
		<a>Data Analysis</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Data/Overview">Overview</a>
			<span>
				<a>New Parts</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Data/GFP">GFP (LVA-tag)</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/T7">T7 (UmuD-tag)</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Antisense">Antisense LuxI</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/ccdB">Celldeath (ccdB)</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/HybridProm">Hybrid Promotor</a>
				</div>
			</span>
                        <span>
				<a>Components</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Input">Input</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Output">Output</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Filter">Filter</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Inverter">Invertimer</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Reset">Reset</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/CellDeath">Cell Death</a>
					<a href="https://2008.igem.org/Team:KULeuven/Data/Memory">Memory</a>
				</div>
			</span>
			<a href="https://2008.igem.org/Team:KULeuven/Data/FullModel">Full Model</a>
		</div>
	</li>
        <li>
		<a>Software</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Software/MultiCell">Multi-cell Toolbox</a>
			<a href="https://2008.igem.org/Team:KULeuven/Software/Simbiology2LaTeX">Simbiology2LaTeX Toolbox</a>
		</div>
	</li>
	<li>
		<a>Notebook</a>
		<div>
			<a href="https://2008.igem.org/Team:KULeuven/Meeting_Calendar">Calendar</a>
                        <a href="https://2008.igem.org/Team:KULeuven/SummerHolidays">Summer Holidays</a>
			<span>
				<a>Reports</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Meeting Reports">Daily</a>
					<a href="https://2008.igem.org/Team:KULeuven/Weekly Meetings">Weekly</a>
				</div>
			</span>
			<span>
				<a>Lab Data</a>
				<div>
					<a href="https://2008.igem.org/Team:KULeuven/Freezer">Freezer</a>
					<a href="https://2008.igem.org/Team:KULeuven/Primers">Primers</a>
					<a href="https://2008.igem.org/Team:KULeuven/Ligation">Ligation</a>
				</div>
			</span>
			<a href="https://2008.igem.org/Team:KULeuven/Tools">Tools</a>
			<a href="https://2008.igem.org/Team:KULeuven/Press">Press</a>
			<a href="https://2008.igem.org/Team:KULeuven/Guestbook">Guestbook</a>
                        
		</div>
	</li>
        
</ul>
</div>
</div>
</html>

Kulbanner.jpg

Submitted Parts

Part number Replaced by Description Type Component
K145000 K145100 eCFP with LVA tag under OmpR control part input
K145001 / T7 RNA polymerase with LVA tag part filter plasmid 2
K145002 / T7 RNA Polymerase with LVA tag under OmpF control composite part filter plasmid 1
K145003 K145102 RiboKey 3d under control of T7 RNA polymerase promotor / /
K145004 K145104 T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. / /
K145005 cI under control of a dual promotor and a RiboLock.
K145006 K145106 Lactonase under PRM control
K145007 K145107 Pulse Generator
K145008 K145108 Constitutive production of LuxR
K145009 K145109 ccdB cell death gene under control of an activating Lux PR
K145010 K145110 Killing switch
K145011 Light sensor to provide dummy input for the system input
K145013 antisense LuxI
K145100 eCFP with LVA tag under control of a OmpF promotor
K145101 Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor.
K145102 RiboKey3d under control of OmpF promotor filter
K145103 T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d
K145104 AND part, T7 and RiboKey3d together will produce LacI. This will repress transcription of LuxI from a pLac promotor inverter
K145105 cI under T7 and PR dual promotor system. Should generate a pulse of cI.
K145106 Lactonase under PRM control
K145107 K145117 Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
K145108 LuxR and TetR under control of a constitutive promotor.
K145109 ccdB cell death gene under the control of a LuxPR promotor.
K145110 Complete cell death mechanism. Combination of BBa_ K145108 and BBa_K145109.
K145111 Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor
K145112 cI under T7 and PR dual promotor system. Should generate a pulse of cI.
K145115 cI under T7 and PR dual promotor system. Should generate a pulse of cI.
K145117 Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present


The Big Sheme of Used Parts

File:THE BIG SCHEME.xls

Retrieved from "http://2008.igem.org/Team:KULeuven/Parts"