Latest revision as of 19:57, 17 July 2008

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Things we did today

Margaret

pSB3K3 was restreaked yesterday, and today I am growing them up in LB media in preparation for a plasmid prep.

checked transformants from yesterday. The controls came out as expected (positive: lawn, negative:lawn(oops, i didn't add selection to the plate)), neither pSB3K3, pSB1A2, nor pSB1AK3 transformed.

I extracted pSB1A2 & pSB1AK3 today with 7uL TE. All 7uL was used to transform DB3.1 cells again.

Last night, I digested the oriT ligation with PstI, left it overnight. This morning, I stopped the digestion, precipitated the DNA and stored it in TE in the -20C freezer. The protocol can be found here.

Margaret, Krystle, Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 19: / Line 19: @@
 ===Name of Task===
-:<strong> name of person/people who performed the task</strong>
+:<strong> Margaret, Krystle, Grace </strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+:* Worked on project summary
-:* e.g. read through papers, worked on proposal, etc.